Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport

Chiba, Kyoko; Araseki, Masahiko; Nozawa, Keisuke et al.

Molecular biology of the cell, 2014, 25(22), 3569-3580

Permalink : https://hdl.handle.net/2115/57645

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論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/57645
タイトル
Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport
著者
Chiba, Kyoko ; Araseki, Masahiko ; Nozawa, Keisuke ; Furukori, Keiko ; Araki, Yoichi ; Matsushima, Takahide ; Nakaya, Tadashi ; Hata, Saori ; Saito, Yuhki ; Uchida, Seiichi ; Okada, Yasushi ; Nairn, Angus C. ; Davis, Roger J. ; Yamamoto, Tohru ; Kinjo, Masataka ; Taru, Hidenori ; Suzuki, Toshiharu
著者名
Chiba, Kyoko
著者名
Araseki, Masahiko
著者名
Nozawa, Keisuke
著者名
Furukori, Keiko
著者名
Araki, Yoichi
著者名
Matsushima, Takahide
e-Rad_Researcher
50374559
著者名
Nakaya, Tadashi
e-Rad_Researcher
40581012
著者名
Hata, Saori
e-Rad_Researcher
70548180
著者名
Saito, Yuhki
e-Rad_Researcher
70315125
著者名
Uchida, Seiichi
e-Rad_Researcher
50272430
著者名
Okada, Yasushi
著者名
Nairn, Angus C.
著者名
Davis, Roger J.
e-Rad_Researcher
10251480
著者名
Yamamoto, Tohru
e-Rad_Researcher
70177971
著者名
Kinjo, Masataka
e-Rad_Researcher
30533731
著者名
Taru, Hidenori
e-Rad_Researcher
80179233
著者名
Suzuki, Toshiharu
言語
英語
発行日
2014-11-05
出版者
American Society for Cell Biology
収録物名
Molecular biology of the cell
巻(号)
25(22)
ページ
3569-3580
抄録
Alzheimer's beta-amyloid precursor protein (APP) associates with kinesin-1 via JNK-interacting protein 1 (JIP1); however, the role of JIP1 in APP transport by kinesin-1 in neurons remains unclear. We performed a quantitative analysis to understand the role of JIP1 in APP axonal transport. In JIP1-deficient neurons, we find that both the fast velocity (similar to 2.7 mu m/s) and high frequency (66%) of anterograde transport of APP cargo are impaired to a reduced velocity (similar to 1.83 mu m/s) and a lower frequency (45%). We identified two novel elements linked to JIP1 function, located in the central region of JIP1b, that interact with the coiled-coil domain of kinesin light chain 1 (KLC1), in addition to the conventional interaction of the JIP1b 11-amino acid C-terminal (C11) region with the tetratricopeptide repeat of KLC1. High frequency of APP anterograde transport is dependent on one of the novel elements in JIP1b. Fast velocity of APP cargo transport requires the C11 domain, which is regulated by the second novel region of JIP1b. Furthermore, efficient APP axonal transport is not influenced by phosphorylation of APP at Thr-668, a site known to be phosphorylated by JNK. Our quantitative analysis indicates that enhanced fast-velocity and efficient high-frequency APP anterograde transport observed in neurons are mediated by novel roles of JIP1b.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
1059-1524
関連情報
PMID
25165140
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1091/mbc.E14-06-1111