Proteomics analysis of heterogeneous flagella in brown algae (Stramenopiles)

Fu, Gang; Nagasato, Chikako; Oka, Seiko; Cock, J. Mark; Motomura, Taizo

Proteomics analysis of heterogeneous flagella in brown algae (Stramenopiles)

Fu, Gang; Nagasato, Chikako; Oka, Seiko et al.

Protist, 2014, 165(5), 662-675

Permalink : https://hdl.handle.net/2115/57666

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/57666
タイトル	Proteomics analysis of heterogeneous flagella in brown algae (Stramenopiles)
著者	Fu, Gang ; Nagasato, Chikako ; Oka, Seiko ; Cock, J. Mark ; Motomura, Taizo
著者名 Fu, Gang
e-Rad_Researcher 00374710 著者名 Nagasato, Chikako
著者名 Oka, Seiko
著者名 Cock, J. Mark
e-Rad_Researcher 30183974 著者名 Motomura, Taizo
言語	英語
キーワード	Blue light receptor
	brown algae
	creatine kinase
	flagella
	phototaxis
	proteomics
発行日	2014-09
出版者	Elsevier
収録物名	Protist
巻(号)	165(5)
ページ	662-675
抄録	Flagella are conserved organelles among eukaryotes and they are composed of many proteins, which are necessary for flagellar assembly, maintenance and function. Stramenopiles, which include brown algae, diatoms and oomycetes, possess two laterally inserted flagella. The anterior flagellum (AF) extends forward and bears tripartite mastigonemes, whilst the smooth posterior flagellum (PF) often has a paraflagellar body structure. These heterogeneous flagella have served as crucial structures in algal studies especially from a viewpoint of phylogeny. However, the protein compositions of the flagella are still largely unknown. Here we report a LC-MS/MS based proteomics analysis of brown algal flagella. In total, 495 flagellar proteins were identified. Functional annotation of the proteome data revealed that brown algal flagellar proteins were associated with cell motility, signal transduction and various metabolic activities. We separately isolated AF and PF and analyzed their protein compositions. This analysis led to the identification of several AF- and PF-specific proteins. Among the PF-specific proteins, we found a candidate novel blue light receptor protein involved in phototaxis, and named it HELMCHROME because of the steering function of PF. Immunological analysis revealed that this protein was localized along the whole length of the PF and concentrated in the paraflagellar body. (C) 2014 Elsevier GmbH. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1434-4610
NCID	AA11281665
関連情報	PMID 25150613
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.protis.2014.07.007