Anchoring protein crystals to mounting loops with hydrogel using inkjet technology

Shinoda, Akira; Tanaka, Yoshikazu; Yao, Min et al.

Acta crystallographica section D : biological crystallography, 2014, 70(11), 2794-2799

Permalink : https://hdl.handle.net/2115/57793

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アクセス権	open access
URI	https://hdl.handle.net/2115/57793
タイトル	Anchoring protein crystals to mounting loops with hydrogel using inkjet technology
著者	Shinoda, Akira ; Tanaka, Yoshikazu ; Yao, Min ; Tanaka, Isao
著者名 Shinoda, Akira
e-Rad_Researcher 20374225 著者名 Tanaka, Yoshikazu
e-Rad_Researcher 40311518 著者名 Yao, Min
e-Rad_Researcher 70093052 著者名 Tanaka, Isao
言語	英語
発行日	2014-11
出版者	Wiley-Blackwell
収録物名	Acta crystallographica section D : biological crystallography
巻(号)	70(11)
ページ	2794-2799
抄録	X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0907-4449
関連情報	PMID 25372671
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1107/S139900471401476X