Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri; Yamamori, Tohru; Niwa, Koichi; Hattori, Yuichi; Kondo, Takashi; Inanami, Osamu

Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri et al.

Biochemical and Biophysical Research Communications, 2015, 456(1), 541-546

Permalink : https://hdl.handle.net/2115/57811

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/57811
タイトル	Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway
著者	Nagane, Masaki ; Yasui, Hironobu ; Sakai, Yuri ; Yamamori, Tohru ; Niwa, Koichi ; Hattori, Yuichi ; Kondo, Takashi ; Inanami, Osamu
著者名 Nagane, Masaki
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
著者名 Sakai, Yuri
e-Rad_Researcher 00512675 著者名 Yamamori, Tohru
e-Rad_Researcher 20301012 著者名 Niwa, Koichi
e-Rad_Researcher 50156361 著者名 Hattori, Yuichi
著者名 Kondo, Takashi
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
言語	英語
キーワード	Ionizing radiation
	endothelial nitric oxide synthase
	ATM
	HSP90
	DNA damage response
発行日	2015-02
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	456(1)
ページ	541-546
抄録	In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
NCID	AA00564395
関連情報	PMID 25498542
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2014.12.002