Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum

Konno, Kohtarou; Matsuda, Keiko; Nakamoto, Chihiro; Uchigashima, Motokazu; Miyazaki, Taisuke; Yamasaki, Miwako; Sakimura, Kenji; Yuzaki, Michisuke; Watanabe, Masahiko

Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum

Konno, Kohtarou; Matsuda, Keiko; Nakamoto, Chihiro et al.

Journal of Neuroscience, 2014, 34(22), 7412-7424

Permalink : https://hdl.handle.net/2115/57866

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アクセス権	open access
URI	https://hdl.handle.net/2115/57866
タイトル	Enriched Expression of GluD1 in Higher Brain Regions and Its Involvement in Parallel Fiber-Interneuron Synapse Formation in the Cerebellum
著者	Konno, Kohtarou ; Matsuda, Keiko ; Nakamoto, Chihiro ; Uchigashima, Motokazu ; Miyazaki, Taisuke ; Yamasaki, Miwako ; Sakimura, Kenji ; Yuzaki, Michisuke ; Watanabe, Masahiko
著者名 Konno, Kohtarou
著者名 Matsuda, Keiko
著者名 Nakamoto, Chihiro
著者名 Uchigashima, Motokazu
e-Rad_Researcher 90374230 著者名 Miyazaki, Taisuke
e-Rad_Researcher 10431305 著者名 Yamasaki, Miwako
著者名 Sakimura, Kenji
著者名 Yuzaki, Michisuke
e-Rad_Researcher 70210945 著者名 Watanabe, Masahiko
言語	英語
キーワード	Cerebellum
	GluD1
	GluR delta 1
	glutamate receptor
	mouse
	synapse
発行日	2014-05-28
出版者	Society for Neuroscience
収録物名	Journal of Neuroscience
巻(号)	34(22)
ページ	7412-7424
抄録	Of the two members of the delta subfamily of ionotropic glutamate receptors, GluD2 is exclusively expressed at parallel fiber-Purkinje cell (PF-PC) synapses in the cerebellum and regulates their structural and functional connectivity. However, little is known to date regarding cellular and synaptic expression of GluD1 and its role in synaptic circuit formation. In the present study, we investigated this issue by producing specific and sensitive histochemical probes for GluD1 and analyzing cerebellar synaptic circuits in GluD1-knock-out mice. GluD1 was widely expressed in the adult mouse brain, with high levels in higher brain regions, including the cerebral cortex, striatum, limbic regions (hippocampus, nucleus accumbens, lateral septum, bed nucleus stria terminalis, lateral habenula, and central nucleus of the amygdala), and cerebellar cortex. In the cerebellar cortex, GluD1 mRNA was expressed at the highest level in molecular layer interneurons and its immunoreactivity was concentrated at PF synapses on interneuron somata. In GluD1-knock-out mice, the density of PF synapses on interneuron somata was significantly reduced and the size and number of interneurons were significantly diminished. Therefore, GluD1 is common to GluD2 in expression at PF synapses, but distinct from GluD2 in neuronal expression in the cerebellar cortex; that is, GluD1 in interneurons and GluD2 in PCs. Furthermore, GluD1 regulates the connectivity of PF-interneuron synapses and promotes the differentiation and/or survival of molecular layer interneurons. These results suggest that GluD1 works in concert with GluD2 for the construction of cerebellar synaptic wiring through distinct neuronal and synaptic expressions and also their shared synapse-connecting function.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0270-6474
EISSN	1529-2401
NCID	AA10620404
関連情報	PMID 24872547
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1523/JNEUROSCI.0628-14.2014