A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter

Kamo, Naoki; Hashiba, Tsuyoshi; Kikukawa, Takashi; Araiso, Tsunehisa; Ihara, Kunio; Nara, Toshifumi

A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter

Kamo, Naoki; Hashiba, Tsuyoshi; Kikukawa, Takashi et al.

Biochemical and Biophysical Research Communications, 2006, 341(2), 285-290

Permalink : https://hdl.handle.net/2115/5787

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アクセス権	open access
URI	https://hdl.handle.net/2115/5787
タイトル	A light-driven proton pump from Haloterrigena turkmenica: Functional expression in Escherichia coli membrane and coupling with a H+ co-transporter
著者	Kamo, Naoki ; Hashiba, Tsuyoshi ; Kikukawa, Takashi ; Araiso, Tsunehisa ; Ihara, Kunio ; Nara, Toshifumi
e-Rad_Researcher 10001976 著者名 Kamo, Naoki 加茂, 直樹
著者名 Hashiba, Tsuyoshi
e-Rad_Researcher 20281842 著者名 Kikukawa, Takashi
e-Rad_Researcher 30151145 著者名 Araiso, Tsunehisa
e-Rad_Researcher 90223297 著者名 Ihara, Kunio
e-Rad_Researcher 30241350 著者名 Nara, Toshifumi
言語	英語
キーワード	Archaeal rhodopsin
	Bacteriorhodopsin
	Light-driven proton pump
	EmrE
	Photocycle
	SMR
	Deltarhodopsin
	Archaerhodopsin
	Multi-drug resistance transporter
発行日	2006-03-10
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	341(2)
ページ	285-290
抄録	A gene encoding putative retinal protein was cloned from Haloterrigena turkmenica (JCM9743). The deduced amino acid sequence was most closely related to that of deltarhodopsin, which functions as a light-driven H+ pump and was identified in a novel strain Haloterrigena sp. arg-4 (K. Ihara, T. Uemura, I. Katagiri, T. Kitajima-Ihara, Y. Sugiyama, Y. Kimura, Y. Mukohata, Evolution of the archaeal rhodopsins: Evolution rate changes by gene duplication and functional differentiation, J. Mol. Biol. 285 (1999) 163–174. GenBank Accession No. AB009620). Thus, we called the present protein H. turkmenica deltarhodopsin (HtdR) in this report. Differing from the Halobacterium salinarum bacteriorhodopsin (bR), functional expression of HtdR was achieved in Escherichia coli membrane with a high yield of 10–15 mg protein/L culture. The photocycle of purified HtdR was similar to that of bR. The photo-induced electrogenic proton pumping activity of HtdR was verified. We co-expressed both HtdR and EmrE, a proton-coupled multi-drug efflux transporter in E. coli, and the cells successfully extruded ethidium, a substrate of EmrE, on illumination.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 16413498
関連情報	URI http://www.sciencedirect.com/science/journal/0006291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2005.12.181