C-ERC/mesothelin provokes lymphatic invasion of colorectal adenocarcinoma

Kawamata, Futoshi; Homma, Shigenori; Kamachi, Hirofumi; Einama, Takahiro; Kato, Yasutaka; Tsuda, Masumi; Tanaka, Shinya; Maeda, Masahiro; Kajino, Kazunori; Hino, Okio; Takahashi, Norihiko; Kamiyama, Toshiya; Nishihara, Hiroshi; Taketomi, Akinobu; Todo, Satoru

C-ERC/mesothelin provokes lymphatic invasion of colorectal adenocarcinoma

Kawamata, Futoshi; Homma, Shigenori; Kamachi, Hirofumi et al.

Journal of gastroenterology, 2014, 49(1), 81-92

Permalink : https://hdl.handle.net/2115/57871

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アクセス権	open access
URI	https://hdl.handle.net/2115/57871
タイトル	C-ERC/mesothelin provokes lymphatic invasion of colorectal adenocarcinoma
著者	Kawamata, Futoshi ; Homma, Shigenori ; Kamachi, Hirofumi ; Einama, Takahiro ; Kato, Yasutaka ; Tsuda, Masumi ; Tanaka, Shinya ; Maeda, Masahiro ; Kajino, Kazunori ; Hino, Okio ; Takahashi, Norihiko ; Kamiyama, Toshiya ; Nishihara, Hiroshi ; Taketomi, Akinobu ; Todo, Satoru
著者名 Kawamata, Futoshi
著者名 Homma, Shigenori
e-Rad_Researcher 60374237 著者名 Kamachi, Hirofumi
著者名 Einama, Takahiro
著者名 Kato, Yasutaka
e-Rad_Researcher 30431307 著者名 Tsuda, Masumi
e-Rad_Researcher 70261287 著者名 Tanaka, Shinya
著者名 Maeda, Masahiro
著者名 Kajino, Kazunori
著者名 Hino, Okio
著者名 Takahashi, Norihiko
e-Rad_Researcher 80322816 著者名 Kamiyama, Toshiya
e-Rad_Researcher 50322805 著者名 Nishihara, Hiroshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
e-Rad_Researcher 60136463 著者名 Todo, Satoru
言語	英語
キーワード	C-ERC/mesothelin
	Colorectal cancer
	Lymphatic invasion
発行日	2014-01
出版者	Springer Japan
収録物名	Journal of gastroenterology
巻(号)	49(1)
ページ	81-92
抄録	Background Lymph node metastasis is a key event of colorectal cancer (CRC) progression. Mesothelin is expressed in various types of malignant tumor and associated with an unfavorable prognosis. The full-length mesothelin (Full-ERC) is cleaved by protease into membrane-bound C-ERC/mesothelin and N-ERC/mesothelin which is secreted into the blood. The aim of this study was to examine the biological role of mesothelin in CRC by clinicopathological analysis and in vitro lymphatic invasion assay. Methods Ninety-one cases of CRC specimens were immunohistochemically examined and the localization of mesothelin in luminal membrane and/or cytoplasm was also evaluated. Lymphatic invasion assay was also performed using the human CRC cell line, WiDr, which was transfected with Full-, N- and C-ERC/mesothelin expression plasmids (Full-WiDr, N-WiDr and C-WiDr). Results Immunohistochemically, "luminal membrane positive" of mesothelin was identified in 37.4 %, and correlated with lymphatic permeation and lymph node metastasis, but not with patients' prognosis. Interestingly, among the patients with lymph node metastasis (N = 38), "luminal membrane positive" of mesothelin significantly correlated with unfavorable patients' outcome. In addition, lymphatic invasion assay revealed that Full-WiDr and C-WiDr more significantly invaded human lymphatic endothelial cells than the Mock-WiDr (P < 0.01). Conclusion The luminal membrane expression of mesothelin was associated with unfavorable prognosis of CRC patients with lymph nodemetastasis. Moreover, this is the first report to prove the biological function of C-ERC/mesothelin associated with lymphatic invasion of cancer in vitro.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0944-1174
NCID	AA10988015
関連情報	PMID 23512344
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00535-013-0773-6