Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene

Han, Xuerong; Satoh, Yasuharu; Kuriki, Yumi; Seino, Teruyuki; Fujita, Shinji; Suda, Takanori; Kobayashi, Takanori; Tajima, Kenji

Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene

Han, Xuerong; Satoh, Yasuharu; Kuriki, Yumi et al.

Journal of bioscience and bioengineering, 2014, 118(5), 514-519

Permalink : https://hdl.handle.net/2115/57954

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アクセス権	open access
URI	https://hdl.handle.net/2115/57954
タイトル	Polyhydroxyalkanoate production by a novel bacterium Massilia sp UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene
著者	Han, Xuerong ; Satoh, Yasuharu ; Kuriki, Yumi ; Seino, Teruyuki ; Fujita, Shinji ; Suda, Takanori ; Kobayashi, Takanori ; Tajima, Kenji
著者名 Han, Xuerong
著者名 Satoh, Yasuharu
著者名 Kuriki, Yumi
著者名 Seino, Teruyuki
著者名 Fujita, Shinji
著者名 Suda, Takanori
著者名 Kobayashi, Takanori
e-Rad_Researcher 00271643 著者名 Tajima, Kenji
言語	英語
キーワード	Polyhydroxyalkanoate
	Seaweed
	Green algae
	Ulva
	Pha locus
	Class I
	Massilia
発行日	2014-11
出版者	Society for Biotechnology, Japan
収録物名	Journal of bioscience and bioengineering
巻(号)	118(5)
ページ	514-519
抄録	We successfully isolated one microorganism (UMI-21) from Ulva, a green algae that contains starch. The strain UMI-21 can produce polyhydroxyalkanoate (PHA) from starch, maltotriose, or maltose as a sole carbon source. Taxonomic studies and 16S rDNA sequence analysis revealed that strain UMI-21 was phylogenetically related to species of the genus Massilia. The PHA content under the cultivation condition using a 10-L jar fermentor was 45.5% (w/w). This value was higher than that obtained after cultivation in a flask, suggesting the possibility of large-scale PHA production by UMI-21 from starch. A major issue for the industrial production of microbial PHAs is the very high production cost. Starch is a relatively inexpensive substrate that is also found in abundant seaweeds such as Ulva. Therefore, the strain isolated in this study may be very useful for producing PHA from seaweeds containing polysaccharides such as starch. In addition, a 3.7-kbp DNA fragment containing the whole PHA synthase gene (phaC) was obtained from the strain UMI-21. The results of open reading frame (ORF) analysis suggested that the DNA fragment contained two ORFs, which were composed of 1740 (phaC) and 564 bp (phaR). The deduced amino acid sequence of PhaC from strain UMI-21 shared high similarity with PhaC from Ralstonia eutropha, which is a representative PHA-producing bacterium with a class I PHA synthase. This is the first report for the cloning of the PHA synthase gene from Massilia species.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1389-1723
関連情報	PMID 24932969
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jbiosc.2014.04.022