Opposing Role of NMDA Receptor GluN2B and GluN2D in Somatosensory Development and Maturation

Yamasaki, Miwako; Okada, Rieko; Takasaki, Chihiro; Toki, Shima; Fukaya, Masahiro; Natsume, Rie; Sakimura, Kenji; Mishina, Masayoshi; Shirakawa, Tetsuo; Watanabe, Masahiko

Opposing Role of NMDA Receptor GluN2B and GluN2D in Somatosensory Development and Maturation

Yamasaki, Miwako; Okada, Rieko; Takasaki, Chihiro et al.

Journal of Neuroscience, 2014, 34(35), 11534-11548

Permalink : https://hdl.handle.net/2115/58008

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アクセス権	open access
URI	https://hdl.handle.net/2115/58008
タイトル	Opposing Role of NMDA Receptor GluN2B and GluN2D in Somatosensory Development and Maturation
著者	Yamasaki, Miwako ; Okada, Rieko ; Takasaki, Chihiro ; Toki, Shima ; Fukaya, Masahiro ; Natsume, Rie ; Sakimura, Kenji ; Mishina, Masayoshi ; Shirakawa, Tetsuo ; Watanabe, Masahiko
e-Rad_Researcher 10431305 著者名 Yamasaki, Miwako
著者名 Okada, Rieko
e-Rad_Researcher 60451449 著者名 Takasaki, Chihiro
e-Rad_Researcher 10312373 著者名 Toki, Shima
e-Rad_Researcher 10360900 著者名 Fukaya, Masahiro
e-Rad_Researcher 60467082 著者名 Natsume, Rie
e-Rad_Researcher 40162325 著者名 Sakimura, Kenji
e-Rad_Researcher 80144351 著者名 Mishina, Masayoshi
e-Rad_Researcher 00187527 著者名 Shirakawa, Tetsuo
e-Rad_Researcher 70210945 著者名 Watanabe, Masahiko
言語	英語
キーワード	barrel
	critical period
	GluN2B
	GluN2D
	NMDA receptor
	somatosensory
発行日	2014-08-27
出版者	Society for Neuroscience
収録物名	Journal of Neuroscience
巻(号)	34(35)
ページ	11534-11548
抄録	Development of correct topographical connections between peripheral receptors and central somatosensory stations requires activity-dependent synapse refinement, in which the NMDA type of glutamate receptors plays a key role. Here we compared functional roles of GluN2B (GluR epsilon 2 or NR2B) and GluN2D (GluR epsilon 4 or NR2D), two major regulatory subunits of neonatal NMDA receptors, in development of whisker-related patterning at trigeminal relay stations. Compared with control littermates, both the appearance of whisker-related patterning and the termination of the critical period, as assessed by unilateral infraorbital nerve transection, were delayed by nearly a day in the somatosensory cortex of GluN2B(+/-) mice but advanced by nearly a day in GluN2D(-/-) mice. Similar temporal shifts were found at subcortical relay stations in the thalamus and brainstem of GluN2B(+/-) and GluN2D(-/-) mice. In comparison, the magnitude of lesion-induced critical period plasticity in the somatosensory cortex, as assessed following row-C whisker removal, was normal in both mutants. Thus, GluN2B and GluN2D play counteractive roles in temporal development and maturation of somatosensory maps without affecting the magnitude of critical period plasticity. To understand the opposing action, we then examined neuronal and synaptic expressions of the two subunits along the trigeminal pathway. At each trigeminal station, GluN2B was predominant at asymmetrical synapses of non-GABAergic neurons, whereas GluN2D was selective to asymmetrical synapses of GABAergic neurons. Together, our findings suggest that GluN2B expressed at glutamatergic synapses on glutamatergic projection neurons facilitates refinement of ascending pathway synapses directly, whereas GluN2D expressed at glutamatergic synapses on GABAergic interneurons delays it indirectly.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
PISSN	0270-6474
関連情報	PMID 25164652
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1523/JNEUROSCI.1811-14.2014