Nitric Oxide Produced During Sublethal Ischemia Is Crucial for the Preconditioning-Induced Down-Regulation of Glutamate Transporter GLT-1 in Neuron/Astrocyte Co-Cultures

Yamada, Takeshi; Kawahara, Koichi; Kosugi, Tatsuro; Tanaka, Motoki

Nitric Oxide Produced During Sublethal Ischemia Is Crucial for the Preconditioning-Induced Down-Regulation of Glutamate Transporter GLT-1 in Neuron/Astrocyte Co-Cultures

Yamada, Takeshi; Kawahara, Koichi; Kosugi, Tatsuro et al.

Neurochemical Research, 2006, 31(1), 49-56

Permalink : https://hdl.handle.net/2115/5814

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アクセス権	open access
URI	https://hdl.handle.net/2115/5814
タイトル	Nitric Oxide Produced During Sublethal Ischemia Is Crucial for the Preconditioning-Induced Down-Regulation of Glutamate Transporter GLT-1 in Neuron/Astrocyte Co-Cultures
著者	Yamada, Takeshi ; Kawahara, Koichi ; Kosugi, Tatsuro ; Tanaka, Motoki
著者名 Yamada, Takeshi
e-Rad_Researcher 20125397 著者名 Kawahara, Koichi 河原, 剛一
著者名 Kosugi, Tatsuro
著者名 Tanaka, Motoki
言語	英語
キーワード	Nitric oxide
	GLT-1
	preconditioning
	ischemic tolerance
発行日	2006-01
出版者	Springer
収録物名	Neurochemical Research
巻(号)	31(1)
ページ	49-56
抄録	In the brain, prior sublethal ischemia (preconditioning, PC) produces tolerance of neurons to subsequent lethal ischemia. This study aims at elucidating whether and how nitric oxide (NO) produced during PC is involved in the PC-induced ischemic tolerance of neurons in neuron/astrocyte co-cultures. The rise in the extracellular concentration of glutamate during ischemia caused by the reversed uptake of glutamate (Glu) by the astrocytic Glu transporter GLT-1 was markedly suppressed by the prior PC treatment, but the suppression was reversed by treatment with an inhibitor of nitric oxide synthase (NOS) during PC. Immunocytochemical and Western blot analyses demonstrated that the expression of GLT-1 was down-regulated after the PC insult, and this down-regulation was also antagonized by treatment with NOS inhibitors during PC. Here we show that nNOS-derived NO produced during PC was crucial for the down-regulation of astrocytic GLT-1, and this down-regulation coincided with an increased survival rate of neurons.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0364-3190
関連情報	PMID 16474996
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11064-005-9077-4