The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

Yambao, Ma. Leonora M.; Masuta, Chikara; Nakahara, Kenji; Uyeda, Ichiro

The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

Yambao, Ma. Leonora M.; Masuta, Chikara; Nakahara, Kenji et al.

Journal of General Virology, 2003, 84, 2861-2869

Permalink : https://hdl.handle.net/2115/5816

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アクセス権	open access
URI	https://hdl.handle.net/2115/5816
タイトル	The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions
著者	Yambao, Ma. Leonora M. ; Masuta, Chikara ; Nakahara, Kenji ; Uyeda, Ichiro
著者名 Yambao, Ma. Leonora M.
e-Rad_Researcher 60281854 著者名 Masuta, Chikara
e-Rad_Researcher 90315606 著者名 Nakahara, Kenji
e-Rad_Researcher 10113523 著者名 Uyeda, Ichiro 上田, 一郎
言語	英語
発行日	2003-10
出版者	Society for General Microbiology
収録物名	Journal of General Virology
巻(号)	84
ページ	2861-2869
抄録	Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigated using a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactions manifested by VPg and HCPro and an interaction between NIb and NIaPro were observed in ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and by in vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not been reported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and the HCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) was important for the VPg–VPg interaction and the central 19 amino acids were needed for the HCPro–VPg interaction.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0022-1317
関連情報	PMID 13679621
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1099/vir.0.19312-0