Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle

Nagatomo, H.; Kagawa, S.; Kishi, Y.; Takuma, T.; Sada, A.; Yamanaka, K.-i.; Abe, Y.; Wada, Y.; Takahashi, M.; Kono, T.; Kawahara, M.

Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle

Nagatomo, H.; Kagawa, S.; Kishi, Y. et al.

Biology of Reproduction, 2013, 88(6), 158

Permalink : https://hdl.handle.net/2115/58264

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アクセス権	open access
URI	https://hdl.handle.net/2115/58264
タイトル	Transcriptional Wiring for Establishing Cell Lineage Specification at the Blastocyst Stage in Cattle
著者	Nagatomo, H. ; Kagawa, S. ; Kishi, Y. ; Takuma, T. ; Sada, A. ; Yamanaka, K.-i. ; Abe, Y. ; Wada, Y. ; Takahashi, M. ; Kono, T. ; Kawahara, M.
著者名 Nagatomo, H.
著者名 Kagawa, S.
著者名 Kishi, Y.
著者名 Takuma, T.
著者名 Sada, A.
著者名 Yamanaka, K.-i.
著者名 Abe, Y.
著者名 Wada, Y.
著者名 Takahashi, M.
著者名 Kono, T.
e-Rad_Researcher 70468700 著者名 Kawahara, M.
言語	英語
キーワード	bovine
	blastocyst
	cell lineage specification
	gene expression
発行日	2013-01-01
出版者	the Society for the Study of Reproduction
収録物名	Biology of Reproduction
巻(号)	88(6)
ページ	158
抄録	Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding reliable transcriptional networks that orchestrate complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative RT-PCR and in situ hybridization using the site-specific cell samples. As a result, we identified 7 ICM-dominant genes and 5 TE-dominant genes not yet found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the preimplantation bovine embryo.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-3363
EISSN	1529-7268
関連情報	PMID 23677984
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1095/biolreprod.113.108993