Selective purification of intestinal maltase complex by affinity chromatography employing an uncompetitive inhibitor as the ligand

Kato, Eisuke; Tsuji, Hiroki; Kawabata, Jun

Tetrahedron, 2015, 71(9), 1419-1424

Permalink : https://hdl.handle.net/2115/58487

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アクセス権	open access
URI	https://hdl.handle.net/2115/58487
タイトル	Selective purification of intestinal maltase complex by affinity chromatography employing an uncompetitive inhibitor as the ligand
著者	Kato, Eisuke ; Tsuji, Hiroki ; Kawabata, Jun
e-Rad_Researcher 40466446 著者名 Kato, Eisuke
著者名 Tsuji, Hiroki
e-Rad_Researcher 60142197 著者名 Kawabata, Jun
言語	英語
キーワード	Affinity chromatography
	Uncompetitive inhibitor
	Glucosidase inhibitor
	Intestinal glucosidase
	Maltase-glucoamylase complex
発行日	2015-03-04
出版者	Elsevier
収録物名	Tetrahedron
巻(号)	71(9)
ページ	1419-1424
抄録	Affinity chromatography of specific enzymes is limited by the presence of related enzymes and the limited selectivity of the affinity ligand. We synthesized and investigated the use of an uncompetitive inhibitor as an affinity ligand, to leverage its three-component interactions. Use of the potent α-glucosidase uncompetitive inhibitor 2-aminoresorcinol as the ligand of the affinity gel offered selective purification of maltase-glucoamylase complex from the crude mixture of intestinal α-glucosidases.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0040-4020
NCID	AA00861787
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tet.2015.01.019