Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing

Alam, Mohammad Zahangir; Bhutto, Abdul Manan; Soomro, Farooq Rahman; Baloch, Javed Hussain; Nakao, Ryo; Kato, Hirotomo; Schoenian, Gabriele; Uezato, Hiroshi; Hashiguchi, Yoshihisa; Katakura, Ken

Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing

Alam, Mohammad Zahangir; Bhutto, Abdul Manan; Soomro, Farooq Rahman et al.

Parasites & Vectors, 2014, 7, 332

Permalink : https://hdl.handle.net/2115/58583

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アクセス権	open access
URI	https://hdl.handle.net/2115/58583
タイトル	Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing
著者	Alam, Mohammad Zahangir ; Bhutto, Abdul Manan ; Soomro, Farooq Rahman ; Baloch, Javed Hussain ; Nakao, Ryo ; Kato, Hirotomo ; Schoenian, Gabriele ; Uezato, Hiroshi ; Hashiguchi, Yoshihisa ; Katakura, Ken
著者名 Alam, Mohammad Zahangir
著者名 Bhutto, Abdul Manan
著者名 Soomro, Farooq Rahman
著者名 Baloch, Javed Hussain
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
e-Rad_Researcher 00346579 著者名 Kato, Hirotomo
著者名 Schoenian, Gabriele
e-Rad_Researcher 60160157 著者名 Uezato, Hiroshi
e-Rad_Researcher 10037385 著者名 Hashiguchi, Yoshihisa
e-Rad_Researcher 10130155 著者名 Katakura, Ken
言語	英語
キーワード	Leishmania (Leishmania) major
	Microsatellite typing
	Population genetics
	Pakistan
発行日	2014-07-16
出版者	Biomed Central
収録物名	Parasites & Vectors
巻(号)	7
ページ	332
抄録	Background: Cutaneous leishmaniasis (CL) is a major and fast increasing public health problem, both among the local Pakistani populations and the Afghan refugees in camps. Leishmania (Leishmania) major is one of the etiological agents responsible for CL in Pakistan. Genetic variability and population structure have been investigated for 66 DNA samples of L. (L.) major isolated from skin biopsy of CL patients. Methods: Multilocus microsatellite typing (MLMT), employing 10 independent genetic markers specific to L. (L.) major, was used to investigate the genetic polymorphisms and population structures of Pakistani L. (L.) major DNA isolated from CL human cases. Their microsatellite profiles were compared to those of 130 previously typed strains of L. (L.) major from various geographical localities. Results: All the markers were polymorphic and fifty-one MLMT profiles were recognized among the 66 L. (L.) major DNA samples. The data displayed significant microsatellite polymorphisms with rare allelic heterozygosities. A Bayesian model-based approach and phylogenetic analysis inferred two L. (L.) major populations in Pakistan. Thirty-four samples belonged to one population and the remaining 32 L. (L.) major samples grouped together into another population. The two Pakistani L. (L.) major populations formed separate clusters, which differ genetically from the populations of L. (L.) major from Central Asia, Iran, Middle East and Africa. Conclusions: The considerable genetic variability of L. (L.) major might be related to the existence of different species of sand fly and/or rodent reservoir host in Sindh province, Pakistan. A comprehensive study of the epidemiology of CL including the situation or spreading of reservoirs and sand fly vectors in these foci is, therefore, warranted.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1756-3305
関連情報	PMID 25030377
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/1756-3305-7-332