open access

https://hdl.handle.net/2115/59169

Effect of Tinospora cordifolia on the reduction of ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells

英語

UV radiation

2014-06-03

Taylor & Francis

Journal of Environmental Science and Health, Part B : Pesticides Food Contaminants and Agricultural Wastes

49(6)

416-421

The safety of Tinospora cordifolia and its potential to protect against ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells were investigated. To evaluate the safety of T. cordifolia, cell viability and agarose gel electrophoresis were carried out using PC12 cells treated with 0 to 100g mL(-1) of methanol extract of T. cordifolia. T. cordifolia extracts did not show cytotoxicity ranging 0 to 100g mL(-1). In addition, T. cordifolia extracts significantly increased cell viability at 1ng, 10ng and 1g mL(-1) concentrations in serum-deprived medium compared to control. To confirm the protective role against UV-induced damage, PC12 cells alone or in the presence of 10ng, 100ng, or 1g mL(-1) of T. cordifolia extract were exposed to 250, 270 and 290nm of UV radiation, which corresponded to doses of 120, 150 and 300 mJ cm(-2), respectively. Treatment with T. cordifolia extracts significantly increased the cell survival rate irradiated at 290nm. In addition, T. cordifolia extracts significantly reduced cyclobutane pyrimidine dimer formation induced by UV irradiation at all wavelengths. In conclusion, T. cordifolia is not toxic and safe for cells. Our findings can support its application as phototherapy in the medical sector.

学術雑誌論文

Accepted Manuscript

This is an Author's Accepted Manuscript of an article published in Journal of Environmental Science and Health, Part B Pesticides, Food Contaminants, and Agricultural Wastes, Vol. 49, Iss. 6, 2014, copyright Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/03601234.2014.894777

0360-1234

AA00697424