Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser

Otomo, Kohei; Hibi, Terumasa; Murata, Takashi et al.

Analytical sciences, 2015, 31(4), 307-313

Permalink : https://hdl.handle.net/2115/59325

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アクセス権
open access
URI
https://hdl.handle.net/2115/59325
タイトル
Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser
著者
Otomo, Kohei ; Hibi, Terumasa ; Murata, Takashi ; Watanabe, Hirotaka ; Kawakami, Ryosuke ; Nakayama, Hiroshi ; Hasebe, Mitsuyasu ; Nemoto, Tomomi
著者名
Otomo, Kohei
e-Rad_Researcher
50554292
著者名
Hibi, Terumasa
e-Rad_Researcher
00242024
著者名
Murata, Takashi
著者名
Watanabe, Hirotaka
e-Rad_Researcher
40508818
著者名
Kawakami, Ryosuke
著者名
Nakayama, Hiroshi
e-Rad_Researcher
40237996
著者名
Hasebe, Mitsuyasu
e-Rad_Researcher
50291084
著者名
Nemoto, Tomomi
言語
英語
キーワード
Two-photon excitation microscopy
multi-point laser scanning method
confocal microscopy
Yb-based laser
発行日
2015-04-10
出版者
Japan Society for Analytical Chemistry
収録物名
Analytical sciences
巻(号)
31(4)
ページ
307-313
抄録
The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
関連情報
PMID
25864674
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.31.307