Three-dimensional imaging of biological cells with picosecond ultrasonics

Danworaphong, Sorasak; Tomoda, Motonobu; Matsumoto, Yuki; Matsuda, Osamu; Ohashi, Toshiro; Watanabe, Hiromu; Nagayama, Masafumi; Gohara, Kazutoshi; Otsuka, Paul H.; Wright, Oliver B.

Three-dimensional imaging of biological cells with picosecond ultrasonics

Danworaphong, Sorasak; Tomoda, Motonobu; Matsumoto, Yuki et al.

Applied physics letters, 2015, 106(16), 163701

Permalink : https://hdl.handle.net/2115/59330

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アクセス権	open access
URI	https://hdl.handle.net/2115/59330
タイトル	Three-dimensional imaging of biological cells with picosecond ultrasonics
著者	Danworaphong, Sorasak ; Tomoda, Motonobu ; Matsumoto, Yuki ; Matsuda, Osamu ; Ohashi, Toshiro ; Watanabe, Hiromu ; Nagayama, Masafumi ; Gohara, Kazutoshi ; Otsuka, Paul H. ; Wright, Oliver B.
著者名 Danworaphong, Sorasak
e-Rad_Researcher 30344485 著者名 Tomoda, Motonobu
著者名 Matsumoto, Yuki
e-Rad_Researcher 30239024 著者名 Matsuda, Osamu
e-Rad_Researcher 30270812 著者名 Ohashi, Toshiro
著者名 Watanabe, Hiromu
e-Rad_Researcher 70374585 著者名 Nagayama, Masafumi
e-Rad_Researcher 40153746 著者名 Gohara, Kazutoshi
著者名 Otsuka, Paul H.
e-Rad_Researcher 90281790 著者名 Wright, Oliver B.
言語	英語
発行日	2015-04-21
出版者	American Institute of Physics
収録物名	Applied physics letters
巻(号)	106(16)
ページ	163701
抄録	We use picosecond ultrasonics to image animal cells in vitro-a bovine aortic endothelial cell and a mouse adipose cell-fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with similar to 1 mu m lateral and similar to 150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Copyright 2015 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 106, 163701, 2015, and may be found at http://dx.doi.org/10.1063/1.4918275
PISSN	0003-6951
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1063/1.4918275