open access

https://hdl.handle.net/2115/59366

A Potential Pathogenic Factor fromMycoplasma hominisis a TLR2-Dependent, Macrophage-Activating, P50-Related Adhesin

英語

macrophage

2014-06-17

Wiley-Blackwell

American Journal of Reproductive Immunology

72(3)

285-295

PROBLEM: Mycoplasma hominis has been implicated in many inflammatory conditions of the human urogenital tract in particular amniotic infections that lead to fetal and neonatal disease and pre-term labor. The mechanisms responsible are poorly defined. METHOD OF STUDY: Biochemical and immunological methods were used to extract, purify, and characterize an inflammatory component present in M. hominis. RESULTS: We isolated and purified to homogeneity a 40-kDa bioactive lipoprotein from M. hominis that was a potent TLR2-dependent, CD14-independent activator of the human THP-1 macrophage cell line. Homology searches of the N-terminal sequence revealed that 22 of the first 23 residues were identical to those seen for the phase-variable M. hominis p50 adhesin. The truncated P50t lipoprotein importantly retained its adhesive properties for human macrophages. CONCLUSION: The unique adhesin/macrophage activator may play a key role in M. hominis infections by triggering an inflammatory cytokine cascade.

学術雑誌論文

Submitted Manuscript Under Review

This is the pre-peer-reviewed version of the following article: FULL CITE, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/aji.12279/abstract;jsessionid=500DAAEB7BE065A3A9874F78C851B78C.f01t02

1046-7408

1600-0897

AA10729786