Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats

Iwabuchi, Sadahiro; Kawahara, Koichi; Makisaka, Koji; Sato, Hideomi

Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats

Iwabuchi, Sadahiro; Kawahara, Koichi; Makisaka, Koji et al.

Experimental Brain Research, 2002, 146(1), 103-116

Permalink : https://hdl.handle.net/2115/5938

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アクセス権	open access
URI	https://hdl.handle.net/2115/5938
タイトル	Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats
著者	Iwabuchi, Sadahiro ; Kawahara, Koichi ; Makisaka, Koji ; Sato, Hideomi
著者名 Iwabuchi, Sadahiro
e-Rad_Researcher 20125397 著者名 Kawahara, Koichi 河原, 剛一
著者名 Makisaka, Koji
著者名 Sato, Hideomi
言語	英語
キーワード	Calcium waves
	Caged ionophore
	Photolysis
	Astrocytes
	Gap junction
発行日	2002-09
出版者	Springer Berlin / Heidelberg
収録物名	Experimental Brain Research
巻(号)	146(1)
ページ	103-116
抄録	Waves of elevated intracellular free calcium that propagate between neighboring astrocytes are important for the intercellular communication between astrocytes as well as between neurons and astrocytes. However, the mechanisms responsible for the initiation and propagation of astrocytic calcium waves remain unclear. In this study, intercellular calcium waves were evoked by focal photolysis of a caged calcium ionophore (DMNPE-caged Br A23187) in cultured astrocytes from newborn rats. The focal photolysis of the caged compound resulted in the increase in intracellular calcium in a single astrocyte, and this increase then propagated to neighboring astrocytes. We also analyzed the spatiotemporal characteristics of the intercellular calcium waves, and estimated the propagation pathways for them. The method using a caged calcium ionophore described in this study provides a new in vitro model for the analysis of intercellular calcium waves.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0014-4819
関連情報	PMID 12192584
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00221-002-1149-y