Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.

Huang, Weiping; Kang, Sung-Sik; Nagai, Katsuhisa; Yanagawa, Yojiro; Takahashi, Yoshiyuki; Nagano, Masashi

Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.

Huang, Weiping; Kang, Sung-Sik; Nagai, Katsuhisa et al.

Reproduction, fertility, and development, 2014, 28(3), 349-356

Permalink : https://hdl.handle.net/2115/59542

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アクセス権	open access
URI	https://hdl.handle.net/2115/59542
タイトル	Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences.
著者	Huang, Weiping ; Kang, Sung-Sik ; Nagai, Katsuhisa ; Yanagawa, Yojiro ; Takahashi, Yoshiyuki ; Nagano, Masashi
著者名 Huang, Weiping
著者名 Kang, Sung-Sik
著者名 Nagai, Katsuhisa
著者名 Yanagawa, Yojiro
e-Rad_Researcher 70167485 著者名 Takahashi, Yoshiyuki
e-Rad_Researcher 70312402 著者名 Nagano, Masashi
言語	英語
キーワード	early antral follicle
	embryo transfer
	IVG
	mitochondria
	ROS
発行日	2014-07-17
出版者	Society for Reproduction and Fertility
収録物名	Reproduction, fertility, and development
巻(号)	28(3)
ページ	349-356
抄録	The objective of this study was to investigate the dynamics of mitochondrial activity in in vitro-grown (IVG) bovine oocytes during pre-maturational culture (pre-IVM) and its relationship to their developmental competence upon being subjected to different pre-IVM durations. After 12-day IVG culture, oocytes were cultured for 0, 10 or 20 h with 3-isobutyl-1-methylxanthine (IBMX) as pre-IVM. Mitochondrial activity in IVG oocytes after 10 h pre-IVM was the highest among all the pre-IVM durations (P < 0.05). In addition, cleavage (79.4%) and blastocyst rates (38.9%) of embryos derived from IVG oocytes with 10 h pre-IVM were higher than those with 20 h pre-IVM (63.0 and 25.8%, respectively; P < 0.05) and similar to those of in vivo-grown oocytes (82.7 and 36.7%, respectively). To confirm the developmental ability of IVG oocytes with 10 h pre-IVM beyond the blastocyst stage in vivo, embryo transfer was attempted. Transferred embryos developed to the elongated embryonic stage (63.6%, 7/11) in the recipient uterus atDay 16 of oestrus, and a male calf was delivered (50%, 1/2). In conclusion, it was indicated that the mitochondrial activity of bovine IVG oocytes peaked at 10 h pre-IVM and was closely correlated with the nuclear maturation and developmental competences of IVG oocytes.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, the journal accepts no responsibility for any errors or omissions it may contain. The definitive version is available at http://dx.doi.org/10.1071/RD14023 © 2014 Society for Reproduction and Fertility.
PISSN	1031-3613
EISSN	1448-5990
NCID	AA10718189
関連情報	PMID 25029376
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1071/RD14023