Analysis of a pair of END+ and END- viruses derived from the same bovine viral diarrhea virus stock reveals the amino acid determinants in N-pro responsible for inhibition of type I interferon production

Kozasa, Takashi; Abe, Yuri; Mitsuhashi, Kazuya; Tamura, Tomokazu; Aoki, Hiroshi; Ishimaru, Masatoshi; Nakamura, Shigeyuki; Okamatsu, Masatoshi; Kida, Hiroshi; Sakoda, Yoshihiro

Analysis of a pair of END+ and END- viruses derived from the same bovine viral diarrhea virus stock reveals the amino acid determinants in N-pro responsible for inhibition of type I interferon production

Kozasa, Takashi; Abe, Yuri; Mitsuhashi, Kazuya et al.

Journal of Veterinary Medical Science, 2015, 77(5), 511-518

Permalink : https://hdl.handle.net/2115/59589

このアイテムのアクセス数:1,196件（2026-08-15 13:54 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
77_14-0420	pdf	1.10 MB	721

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/59589
タイトル	Analysis of a pair of END+ and END- viruses derived from the same bovine viral diarrhea virus stock reveals the amino acid determinants in N-pro responsible for inhibition of type I interferon production
著者	Kozasa, Takashi ; Abe, Yuri ; Mitsuhashi, Kazuya ; Tamura, Tomokazu ; Aoki, Hiroshi ; Ishimaru, Masatoshi ; Nakamura, Shigeyuki ; Okamatsu, Masatoshi ; Kida, Hiroshi ; Sakoda, Yoshihiro
e-Rad_Researcher 90263368 著者名 Kozasa, Takashi
著者名 Abe, Yuri
著者名 Mitsuhashi, Kazuya
著者名 Tamura, Tomokazu
e-Rad_Researcher 10440067 著者名 Aoki, Hiroshi
著者名 Ishimaru, Masatoshi
著者名 Nakamura, Shigeyuki
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
言語	英語
キーワード	bovine viral diarrhea virus
	END phenomenon
	interferon regulatory factor-3
	N-pro
	type I interferon
発行日	2015-05
出版者	日本獣医学会
出版者	The Japanese Society of Veterinary Science
収録物名	Journal of Veterinary Medical Science
巻(号)	77(5)
ページ	511-518
抄録	The Exaltation of Newcastle disease virus (END) phenomenon is induced by the inhibition of type I interferon in pestivirus-infected cells in vitro, via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the property of the viral autoprotease protein N-pro. Reportedly, the amino acid residues in the zinc-binding TRASH motif of N-pro determine the difference in characteristics between END-phenomenon-positive (END) and END-phenomenon-negative (END-) classical swine fever viruses (CSFVs). However, the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not been elucidated from the genomic differences between END+ and END- viruses using reverse genetics till date. In the present study, comparison of complete genome sequences of a pair of END+ and END- viruses isolated from the same virus stock revealed that there were only four amino acid substitutions (D136G, I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with and without mutations at these positions were generated using reverse genetics. The END assay, measurements of induced type I interferon and IRF-3 detection in cells infected with these viruses revealed that the aspartic acid at position 136 in the zinc-binding TRASH motif of N-pro was required to inhibit the production of type I interferon via the degradation of cellular IRF-3, consistently with CSFV.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
PISSN	0916-7250
NCID	AA10796138
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.14-0420