Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells

Motegi, Hiroaki; Kamoshima, Yuuta; Terasaka, Shunsuke; Kobayashi, Hiroyuki; Houkin, Kiyohiro

Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells

Motegi, Hiroaki; Kamoshima, Yuuta; Terasaka, Shunsuke et al.

Neuropathology, 2014, 34(4), 378-385

Permalink : https://hdl.handle.net/2115/59747

このアイテムのアクセス数:1,113件（2026-10-16 17:03 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Collagen1CD133Neuropathology_HUSCAP	pdf	2.37 MB	1,325

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/59747
タイトル	Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells
著者	Motegi, Hiroaki ; Kamoshima, Yuuta ; Terasaka, Shunsuke ; Kobayashi, Hiroyuki ; Houkin, Kiyohiro
著者名 Motegi, Hiroaki
著者名 Kamoshima, Yuuta
e-Rad_Researcher 10447055 著者名 Terasaka, Shunsuke
著者名 Kobayashi, Hiroyuki
e-Rad_Researcher 90229146 著者名 Houkin, Kiyohiro
言語	英語
キーワード	CD133
	glioblastoma
	stem cell niche
	stem cell
	type 1 collagen
発行日	2014-08
出版者	Wiley-Blackwell
収録物名	Neuropathology
巻(号)	34(4)
ページ	378-385
抄録	Cancer stem cells are thought to be closely related to tumor progression and recurrence, making them attractive therapeutic targets. Stem cells of various tissues exist within niches maintaining their stemness. Glioblastoma stem cells (GSCs) are located at tumor capillaries and the perivascular niche, which are considered to have an important role in maintaining GSCs. There were some extracellular matrices (ECM) on the perivascular connective tissue, including type 1 collagen. We here evaluated whether type 1 collagen has a potential niche for GSCs. Imunohistochemical staining of type 1 collagen and CD133, one of the GSCs markers, on glioblastoma (GBM) tissues showed CD133-positive cells were located in immediate proximity to type 1 collagen around tumor vessels. We cultured human GBM cell lines, U87MG and GBM cells obtained from fresh surgical tissues, T472 and T555, with serum-containing medium (SCM) or serum-free medium with some growth factors (SFM) and in non-coated (Non-coat) or type 1 collagen-coated plates (Col). The RNA expression levels of CD133 and Nestin as stem cell markers in each condition were examined. The Col condition not only with SFM but SCM made GBM cells more enhanced in RNA expression of CD133, compared to Non-coat/SCM. Semi-quantitative measurement of CD133-positive cells by immunocytochemistry showed a statistically significant increase of CD133-positive cells in Col/SFM. In addition, T472 cell line cultured in the Col/SFM had capabilities of sphere formation and tumorigenesis. Type 1 collagen was found in the perivascular area and showed a possibility to maintain GSCs. These findings suggest that type 1 collagen could be one important niche component for CD133-positive GSCs and maintain GSCs in adherent culture.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the accepted version of the following article: Motegi, H., Kamoshima, Y., Terasaka, S., Kobayashi, H. and Houkin, K. (2014), Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells. Neuropathology, 34: 378–385, which has been published in final form at http://dx.doi.org/10.1111/neup.12117
PISSN	0919-6544
関連情報	PMID 24673436
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/neup.12117