Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies

Amengual, Olga; Horita, Tetsuya; Binder, Walter; Norman, Gary L.; Shums, Zakera; Kato, Masaru; Otomo, Kotaro; Fujieda, Yuichiro; Oku, Kenji; Bohgaki, Toshiyuki; Yasuda, Shinsuke; Atsumi, Tatsuya

Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies

Amengual, Olga; Horita, Tetsuya; Binder, Walter et al.

Rheumatology international, 2014, 34(9), 1225-1230

Permalink : https://hdl.handle.net/2115/59853

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アクセス権	open access
URI	https://hdl.handle.net/2115/59853
タイトル	Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies
著者	Amengual, Olga ; Horita, Tetsuya ; Binder, Walter ; Norman, Gary L. ; Shums, Zakera ; Kato, Masaru ; Otomo, Kotaro ; Fujieda, Yuichiro ; Oku, Kenji ; Bohgaki, Toshiyuki ; Yasuda, Shinsuke ; Atsumi, Tatsuya
e-Rad_Researcher 10636939 著者名 Amengual, Olga
e-Rad_Researcher 50399929 著者名 Horita, Tetsuya
著者名 Binder, Walter
著者名 Norman, Gary L.
著者名 Shums, Zakera
e-Rad_Researcher 10755896 著者名 Kato, Masaru
著者名 Otomo, Kotaro
著者名 Fujieda, Yuichiro
著者名 Oku, Kenji
著者名 Bohgaki, Toshiyuki
e-Rad_Researcher 00374231 著者名 Yasuda, Shinsuke
e-Rad_Researcher 20301905 著者名 Atsumi, Tatsuya
言語	英語
キーワード	Antiphospholipid syndrome
	Antiphospholipid antibodies
	Thrombosis
	Lupus anticoagulant
発行日	2014-09
出版者	Springer
収録物名	Rheumatology international
巻(号)	34(9)
ページ	1225-1230
抄録	Phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) were strongly correlated with the presence of lupus anticoagulant showing a high specificity for the diagnosis of antiphospholipid syndrome. However, the main criticism for the clinical applicability of aPS/PT testing is the lack of reproducibility of the results among laboratories. In this study, we measured IgG and IgM aPS/PT using our original in-house enzyme-linked immunosorbent assays (ELISA) and commercial ELISA kits to assess the assay performance and to evaluate the accuracy of aPS/PT results. The study included 111 plasma samples collected from patients and stored at our laboratory for aPS/PT assessment. Sixty-one samples were tested for IgG aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgG ELISA kit (INOVA Diagnostics, Inc., USA). Fifty samples were evaluated for IgM aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgM ELISA kit (INOVA Diagnostics). Ninety-eight percent of samples yielded concordant results for IgG aPS/PT and 82 % for IgM aPS/PT. There was an excellent agreement between the IgG aPS/PT assays (Cohen kappa = 0.962) and moderate agreement between the IgM aPS/PT assays (kappa = 0.597). Statistically significant correlations in the aPS/PT results were obtained from both IgG and IgM aPS/PT assays (r = 0.749, r = 0.622, p < 0.001, respectively). In conclusion, IgG and IgM detection by ELISA is accurate. The performance of aPS/PT is reliable, and concordant results can be obtained using different ELISA methods.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0172-8172
関連情報	PMID 24497039
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00296-014-2951-0