SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

Kawaguchi, Tetsuya; Tanigawa, Akie; Naganuma, Takao et al.

Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(14), 4304-4309

Permalink : https://hdl.handle.net/2115/59917

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アクセス権
open access
URI
https://hdl.handle.net/2115/59917
タイトル
SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies
著者
Kawaguchi, Tetsuya ; Tanigawa, Akie ; Naganuma, Takao ; Ohkawa, Yasuyuki ; Souquere, Sylvie ; Pierron, Gerard ; Hirose, Tetsuro
著者名
Kawaguchi, Tetsuya
著者名
Tanigawa, Akie
著者名
Naganuma, Takao
著者名
Ohkawa, Yasuyuki
著者名
Souquere, Sylvie
著者名
Pierron, Gerard
著者名
Hirose, Tetsuro
言語
英語
キーワード
chromatin-remodeling complex
long noncoding RNA
nuclear bodies
ribonucleoprotein assembly
発行日
2015-04-08
出版者
The National Academy of Sciences
収録物名
Proceedings of the National Academy of Sciences of the United States of America
巻(号)
112(14)
ページ
4304-4309
記述
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1423819112/-/DCSupplemental.
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1423819112/-/DCSupplemental
抄録
Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. EM observations revealed that SWI/SNF complexes were enriched in paraspeckle subdomains depleted of chromatin. Knockdown of SWI/SNF components resulted in paraspeckle disintegration, but mutation of the ATPase domain of the catalytic subunit BRG1 did not affect paraspeckle integrity, indicating that the essential role of SWI/SNF complexes in paraspeckle formation does not require their canonical activity. Knockdown of SWI/SNF complexes barely affected the levels of known essential paraspeckle components, but markedly diminished the interactions between essential PSPs, suggesting that SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. The interactions between SWI/SNF components and essential PSPs were maintained in NEAT1-depleted cells, suggesting that SWI/SNF complexes not only facilitate interactions between PSPs, but also recruit PSPs during paraspeckle assembly. SWI/SNF complexes were also required for Satellite III lncRNA-dependent formation of nuclear stress bodies under heat-shock conditions. Our data suggest the existence of a common mechanism underlying the formation of lncRNA-dependent nuclear body architectures in mammalian cells.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0027-8424
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1073/pnas.1423819112