A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains

Nao, Naganori; Kajihara, Masahiro; Manzoor, Rashid; Maruyama, Junki; Yoshida, Reiko; Muramatsu, Mieko; Miyamoto, Hiroko; Igarashi, Manabu; Eguchi, Nao; Sato, Masahiro; Kondoh, Tatsunari; Okamatsu, Masatoshi; Sakoda, Yoshihiro; Kida, Hiroshi; Takada, Ayato

A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains

Nao, Naganori; Kajihara, Masahiro; Manzoor, Rashid et al.

PLoS ONE, 2015, 10(9), e0137989

Permalink : https://hdl.handle.net/2115/60254

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アクセス権	open access
URI	https://hdl.handle.net/2115/60254
タイトル	A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains
著者	Nao, Naganori ; Kajihara, Masahiro ; Manzoor, Rashid ; Maruyama, Junki ; Yoshida, Reiko ; Muramatsu, Mieko ; Miyamoto, Hiroko ; Igarashi, Manabu ; Eguchi, Nao ; Sato, Masahiro ; Kondoh, Tatsunari ; Okamatsu, Masatoshi ; Sakoda, Yoshihiro ; Kida, Hiroshi ; Takada, Ayato
著者名 Nao, Naganori
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
著者名 Manzoor, Rashid
著者名 Maruyama, Junki
e-Rad_Researcher 80435966 著者名 Yoshida, Reiko
著者名 Muramatsu, Mieko
著者名 Miyamoto, Hiroko
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
著者名 Eguchi, Nao
著者名 Sato, Masahiro
著者名 Kondoh, Tatsunari
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
発行日	2015-09-14
出版者	Public Library of Science
収録物名	PLoS ONE
巻(号)	10(9)
ページ	e0137989
抄録	Two highly pathogenic avian influenza virus strains, A/duck/Hokkaido/WZ83/2010 ( H5N1) (WZ83) and A/duck/Hokkaido/WZ101/2010 (H5N1) (WZ101), which were isolated from wild ducks in Japan, were found to be genetically similar, with only two amino acid differences in their M1 and PB1 proteins at positions 43 and 317, respectively. We found that both WZ83 and WZ101 caused lethal infection in chickens but WZ101 killed them more rapidly than WZ83. Interestingly, ducks experimentally infected with WZ83 showed no or only mild clinical symptoms, whereas WZ101 was highly lethal. We then generated reassortants between these viruses and found that exchange of the M gene segment completely switched the pathogenic phenotype in both chickens and ducks, indicating that the difference in the pathogenicity for these avian species between WZ83 and WZ101 was determined by only a single amino acid in the M1 protein. It was also found that WZ101 showed higher pathogenicity than WZ83 in mice and that WZ83, whose M gene was replaced with that of WZ101, showed higher pathogenicity than wild-type WZ83, although this reassortant virus was not fully pathogenic compared to wild-type WZ101. These results suggest that the amino acid at position 43 of the M1 protein is one of the factors contributing to the pathogenicity of H5N1 highly pathogenic avian influenza viruses in both avian and mammalian hosts.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0137989