Protozoal ciliate promotes bacterial autoinducer-2 accumulation in mixed culture with Escherichia coli

Oguri, Satoshi; Hanawa, Tomoko; Matsuo, Junji; Ishida, Kasumi; Yamazaki, Tomohiro; Nakamura, Shinji; Okubo, Torahiko; Fukumoto, Tatsuya; Akizawa, Kouzi; Shimizu, Chikara; Kamiya, Shigeru; Yamaguchi, Hiroyuki

Protozoal ciliate promotes bacterial autoinducer-2 accumulation in mixed culture with Escherichia coli

Oguri, Satoshi; Hanawa, Tomoko; Matsuo, Junji et al.

The Journal of General and Applied Microbiology, 2015, 61(5), 203-210

Permalink : https://hdl.handle.net/2115/60277

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アクセス権	open access
URI	https://hdl.handle.net/2115/60277
タイトル	Protozoal ciliate promotes bacterial autoinducer-2 accumulation in mixed culture with Escherichia coli
著者	Oguri, Satoshi ; Hanawa, Tomoko ; Matsuo, Junji ; Ishida, Kasumi ; Yamazaki, Tomohiro ; Nakamura, Shinji ; Okubo, Torahiko ; Fukumoto, Tatsuya ; Akizawa, Kouzi ; Shimizu, Chikara ; Kamiya, Shigeru ; Yamaguchi, Hiroyuki
著者名 Oguri, Satoshi
著者名 Hanawa, Tomoko
e-Rad_Researcher 50359486 著者名 Matsuo, Junji
著者名 Ishida, Kasumi
著者名 Yamazaki, Tomohiro
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
著者名 Okubo, Torahiko
著者名 Fukumoto, Tatsuya
著者名 Akizawa, Kouzi
e-Rad_Researcher 00292029 著者名 Shimizu, Chikara
著者名 Kamiya, Shigeru
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	autoinducer-2
	ciliates
	Escherichia coli
	luxS
	quorum sensing
	Tetrahymena thermophila
	vacuole
	ydgG
発行日	2015
出版者	応用微生物学・分子細胞生物学研究奨励会
収録物名	The Journal of General and Applied Microbiology
巻(号)	61(5)
ページ	203-210
抄録	We have previously demonstrated conjugation of Escherichia coli into vacuoles of the protozoal ciliate (Tetrahymena thermophila). This indicated a possible role of ciliates in evoking bacterial quorum sensing, directly connecting bacterial survival via accumulation in the ciliate vacuoles. We therefore assessed if ciliates promoted bacterial autoinducer (AI)-2 accumulation with vacuole formation, which controls quorum sensing. E. coli AI-2 accumulation was significantly enhanced in the supernatants of a mixed culture of ciliates and bacteria, likely depending on ciliate density rather than bacterial concentration. As expected, AI-2 production was significantly correlated with vacuole formation. The experiment with E. coli luxS mutants showed that ciliates failed to enhance bacterial AI-2 accumulation, denying a nonspecific phenomenon. Fluorescence microscopy revealed accumulation of fragmented bacteria in ciliate vacuoles, and, more importantly, expulsion of the vacuoles containing disrupted bacteria into the culture supernatant. There was no increase in the expression of luxS (encoding AI-2) or ydgG (a transporter for controlling bacterial export of AI-2). We conclude that ciliates promote bacterial AI-2 accumulation in a mixed culture, via accumulation of disrupted bacteria in ciliate vacuoles followed by expulsion of the vacuoles, independently of luxS or ydgG gene induction. This is believed to be the first demonstration of a relationship between E. coli AI-2 dynamics and ciliates. In the natural environment, ciliate biotopes may provide a survival advantage to bacteria inhabiting such biotopes, via evoking quorum sensing.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	©2015 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
PISSN	0022-1260
EISSN	1349-8037
NCID	AA00698664
関連情報	PMID 26582290
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2323/jgam.61.203