Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

Yamamoto, Tsuneyuki; Yamamoto, Tomomaya; Yamada, Tamaki et al.

Histochemistry and cell biology, 2014, 142(5), 489-496

Permalink : https://hdl.handle.net/2115/60279

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/60279
タイトル
Hertwig's epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars
著者
Yamamoto, Tsuneyuki ; Yamamoto, Tomomaya ; Yamada, Tamaki ; Hasegawa, Tomoka ; Hongo, Hiromi ; Oda, Kimimitsu ; Amizuka, Norio
e-Rad_Researcher
80200822
著者名
Yamamoto, Tsuneyuki
著者名
Yamamoto, Tomomaya
著者名
Yamada, Tamaki
著者名
Hasegawa, Tomoka
著者名
Hongo, Hiromi
著者名
Oda, Kimimitsu
e-Rad_Researcher
30242431
著者名
Amizuka, Norio
言語
英語
キーワード
Hertwig's epithelial root sheath
Cementoblasts
Epithelial-mesenchymal transition
Acellular cementum
Rat molars
発行日
2014-11
出版者
Springer
収録物名
Histochemistry and cell biology
巻(号)
142(5)
ページ
489-496
抄録
This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig's epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial-mesenchymal transition (EMT). Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis. In brief, this study supports the notion that cementoblasts derive from the dental follicle.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The final publication is available at link.springer.com
PISSN
0948-6143
NCID
AA11033302
関連情報
PMID
24859538
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s00418-014-1230-1