CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing

Kubota, Yoshimasa; Kawakami, Hiroshi; Natsuizaka, Mitsuteru; Kawakubo, Kazumichi; Marukawa, Katsuji; Kudo, Taiki; Abe, Yoko; Kubo, Kimitoshi; Kuwatani, Masaki; Hatanaka, Yutaka; Mitsuhashi, Tomoko; Matsuno, Yoshihiro; Sakamoto, Naoya

CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing

Kubota, Yoshimasa; Kawakami, Hiroshi; Natsuizaka, Mitsuteru et al.

Journal of Gastroenterology, 2015, 50(2), 203-210

Permalink : https://hdl.handle.net/2115/60616

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アクセス権	open access
URI	https://hdl.handle.net/2115/60616
タイトル	CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing
著者	Kubota, Yoshimasa ; Kawakami, Hiroshi ; Natsuizaka, Mitsuteru ; Kawakubo, Kazumichi ; Marukawa, Katsuji ; Kudo, Taiki ; Abe, Yoko ; Kubo, Kimitoshi ; Kuwatani, Masaki ; Hatanaka, Yutaka ; Mitsuhashi, Tomoko ; Matsuno, Yoshihiro ; Sakamoto, Naoya
著者名 Kubota, Yoshimasa
e-Rad_Researcher 80399823 著者名 Kawakami, Hiroshi
著者名 Natsuizaka, Mitsuteru
著者名 Kawakubo, Kazumichi
著者名 Marukawa, Katsuji
著者名 Kudo, Taiki
著者名 Abe, Yoko
著者名 Kubo, Kimitoshi
e-Rad_Researcher 50431375 著者名 Kuwatani, Masaki
e-Rad_Researcher 30589924 著者名 Hatanaka, Yutaka
e-Rad_Researcher 60348208 著者名 Mitsuhashi, Tomoko
著者名 Matsuno, Yoshihiro
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	CTNNB1
	Solid-pseudopapillary neoplasms of the pancreas
	Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA)
	Next-generation sequencing
発行日	2015-02
出版者	Springer
収録物名	Journal of Gastroenterology
巻(号)	50(2)
ページ	203-210
抄録	Solid-pseudopapillary neoplasm (SPN), a rare neoplasm of the pancreas, frequently harbors mutations in exon 3 of the cadherin-associated protein beta 1 (CTNNB1) gene. Here, we analyzed SPN tissue for CTNNB1 mutations by deep sequencing using next-generation sequencing (NGS). Tissue samples from 7 SPNs and 31 other pancreatic lesions (16 pancreatic ductal adenocarcinomas (PDAC), 11 pancreatic neuroendocrine tumors (PNET), 1 acinar cell carcinoma, 1 autoimmune pancreatitis lesion, and 2 focal pancreatitis lesions) were analyzed by NGS for mutations in exon 3 of CTNNB1. A single-base-pair missense mutations in exon 3 of CTNNB1 was observed in all 7 SPNs and in 1 of 11 PNET samples. However, mutations were not observed in the tissue samples of any of the 16 PDAC or other four pancreatic disease cases. The variant frequency of CTNNB1 ranged from 5.4 to 48.8 %. Mutational analysis of CTNNB1 by NGS is feasible and was achieved using SPN samples obtained by endoscopic ultrasound-guided fine needle aspiration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0944-1174
関連情報	PMID 24700283
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00535-014-0954-y