open access

https://hdl.handle.net/2115/60635

Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing

英語

Obligate biotroph

2015

springer

Methods in Molecular Biology

1236

171-180

Fungal viruses (mycoviruses) often have a significant impact not only on phenotypic expression of the host fungus but also on higher order biological interactions, e.g., conferring plant stress tolerance via an endophytic host fungus. Arbuscular mycorrhizal (AM) fungi in the phylum Glomeromycota associate with most land plants and supply mineral nutrients to the host plants. So far, little information about mycoviruses has been obtained in the fungi due to their obligate biotrophic nature. Here we provide a technical breakthrough, “two-step strategy” in combination with deep-sequencing, for virological study in AM fungi; dsRNA is first extracted and sequenced using material obtained from highly productive open pot culture, and then the presence of viruses is verified using pure material produced in the in vitro monoxenic culture. This approach enabled us to demonstrate the presence of several viruses for the first time from a glomeromycotan fungus.

学術雑誌論文

Accepted Manuscript

The final publication is available at www.springerlink.com

1064-3745