Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein

Uyeda, I.; Suga, H.; Lee, SY et al.

Journal of General Virology, 1995, 76, 975-978

Permalink : https://hdl.handle.net/2115/6064

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URI	https://hdl.handle.net/2115/6064
タイトル	Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein
著者	Uyeda, I. ; Suga, H. ; Lee, SY ; Yan, J. ; Hataya, T. ; Kimura, I. ; Shikata, E.
e-Rad_Researcher 10113523 著者名 Uyeda, I. 上田, 一郎
著者名 Suga, H.
著者名 Lee, SY
著者名 Yan, J.
著者名 Hataya, T.
著者名 Kimura, I.
著者名 Shikata, E.
言語	英語
発行日	1995-05
出版者	Society for General Microbiology
収録物名	Journal of General Virology
巻(号)	76
ページ	975-978
抄録	The complete nucleotide sequence of rice ragged stunt virus genome segment 9(S9) was determined. The S9 segment is 1132 nucleotides long and has a long open reading frame starting from the first AUG codon at nucleotide position 14-16 and terminating at a UAG codon located at 1028-1030, which could encode a polypeptide with an Mr of 38 600 (P9). The encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. An immunological study demonstrated that P9 was the smallest of the structural proteins. The P9 polypeptide was expressed as a fusion protein with maltose binding protein in Escherichia coli. Antisera to purified virions and to the fusion protein reacted with both the bacterially expressed polypeptide and the smallest polypeptide of the purified virus in immunoblotting analyses.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0022-1317