Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice

Konno, Kohtarou; Takahashi-Iwanaga, Hiromi; Uchigashima, Motokazu; Miyasaka, Kyoko; Funakoshi, Akihiro; Watanabe, Masahiko; Iwanaga, Toshihiko

Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice

Konno, Kohtarou; Takahashi-Iwanaga, Hiromi; Uchigashima, Motokazu et al.

Histochemistry and Cell Biology, 2015, 143(3), 301-312

Permalink : https://hdl.handle.net/2115/60738

このアイテムのアクセス数:1,252件（2026-08-12 03:44 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
2014_HCB_revise	pdf	2.72 MB	1,266

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/60738
タイトル	Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice
著者	Konno, Kohtarou ; Takahashi-Iwanaga, Hiromi ; Uchigashima, Motokazu ; Miyasaka, Kyoko ; Funakoshi, Akihiro ; Watanabe, Masahiko ; Iwanaga, Toshihiko
著者名 Konno, Kohtarou
著者名 Takahashi-Iwanaga, Hiromi
e-Rad_Researcher 10614662 著者名 Uchigashima, Motokazu
e-Rad_Researcher 90166140 著者名 Miyasaka, Kyoko
著者名 Funakoshi, Akihiro
e-Rad_Researcher 70210945 著者名 Watanabe, Masahiko
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
言語	英語
キーワード	Cholecystokinin
	CCK1 receptor
	Pancreas
	Stomach
	Gallbladder
発行日	2015-03
出版者	Springer
収録物名	Histochemistry and Cell Biology
巻(号)	143(3)
ページ	301-312
抄録	Information concerning the cellular localization of cholecystokinin (CCK)-1 receptors has been discrepant and remained scanty at ultrastructural levels. The present immunohistochemical study at light and electron microscopic levels revealed the distinct localization of CCK1 receptors in visceral organs. Immunohistochemistry by use of a purified antibody against mouse CCK1 receptor was applied to fixed tissue sections of the pancreas, gallbladder, stomach, and intestine of mice. A silver-intensified immunogold method revealed the subcellular localization under electron microscope. The immunoreactivity for CCK1 receptors was selectively found in the basolateral membrane of pancreatic acinar cells and gastric chief cells but was absent in pancreatic islets and gastric D cells. Another intense expression in the gut was seen in the myenteric nerve plexus of the antro-duodenal region and some populations of c-Kit-expressing pacemaker cells in the duodenal musculature. The gallbladder contained smooth muscle fibers with an intense immunoreactivity of CCK1 receptors on cell surfaces. The restricted localization of CCK1 receptors on the basolateral membrane of pancreatic acinar cells and gastric chief cells, along with their absence in the islets of Langerhans and gastric D cells, provides definitive information concerning the regulatory mechanism by circulating CCK. Especially, the subcellular localization in the acinar cells completes the investigation for the detection of circulating CCK by the basolateral membrane.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	0948-6143
関連情報	PMID 25249350
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00418-014-1281-3