A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog

Zhao, Chen; Takeshima, Ryoma; Zhu, Jianghui; Xu, Meilan; Sato, Masako; Watanabe, Satoshi; Kanazawa, Akira; Liu, Baohui; Kong, Fanjiang; Yamada, Tetsuya; Abe, Jun

A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog

Zhao, Chen; Takeshima, Ryoma; Zhu, Jianghui et al.

BMC plant biology, 2016, 16, 20

Permalink : https://hdl.handle.net/2115/60741

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アクセス権	open access
URI	https://hdl.handle.net/2115/60741
タイトル	A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog
著者	Zhao, Chen ; Takeshima, Ryoma ; Zhu, Jianghui ; Xu, Meilan ; Sato, Masako ; Watanabe, Satoshi ; Kanazawa, Akira ; Liu, Baohui ; Kong, Fanjiang ; Yamada, Tetsuya ; Abe, Jun
著者名 Zhao, Chen
著者名 Takeshima, Ryoma
著者名 Zhu, Jianghui
著者名 Xu, Meilan
著者名 Sato, Masako
著者名 Watanabe, Satoshi
e-Rad_Researcher 30281794 著者名 Kanazawa, Akira
著者名 Liu, Baohui
著者名 Kong, Fanjiang
e-Rad_Researcher 70374618 著者名 Yamada, Tetsuya
e-Rad_Researcher 00192998 著者名 Abe, Jun
言語	英語
キーワード	Maturity gene E9
	FLOWERING LOCUS T
	FT2a
	Soybean (Glycine max)
	Flowering
	Ty1/copia-like retrotransposon
	SORE-1
	Methylation
発行日	2016-01-19
出版者	BioMed Central
収録物名	BMC plant biology
巻(号)	16
ページ	20
抄録	Background: Understanding the molecular mechanisms of flowering and maturity is important for improving the adaptability and yield of seed crops in different environments. In soybean, a facultative short-day plant, genetic variation at four maturity genes, E1 to E4, plays an important role in adaptation to environments with different photoperiods. However, the molecular basis of natural variation in time to flowering and maturity is poorly understood. Using a cross between early-maturing soybean cultivars, we performed a genetic and molecular study of flowering genes. The progeny of this cross segregated for two maturity loci, E1 and E9. The latter locus was subjected to detailed molecular analysis to identify the responsible gene. Results: Fine mapping, sequencing, and expression analysis revealed that E9 is FT2a, an ortholog of Arabidopsis FLOWERING LOCUS T. Regardless of daylength conditions, the e9 allele was transcribed at a very low level in comparison with the E9 allele and delayed flowering. Despite identical coding sequences, a number of single nucleotide polymorphisms and insertions/deletions were detected in the promoter, untranslated regions, and introns between the two cultivars. Furthermore, the e9 allele had a Ty1/copia-like retrotransposon, SORE-1, inserted in the first intron. Comparison of the expression levels of different alleles among near-isogenic lines and photoperiod-insensitive cultivars indicated that the SORE-1 insertion attenuated FT2a expression by its allele-specific transcriptional repression. SORE-1 was highly methylated, and did not appear to disrupt FT2a RNA processing. Conclusions: The soybean maturity gene E9 is FT2a, and its recessive allele delays flowering because of lower transcript abundance that is caused by allele-specific transcriptional repression due to the insertion of SORE-1. The FT2a transcript abundance is thus directly associated with the variation in flowering time in soybean. The e9 allele may maintain vegetative growth in early-flowering genetic backgrounds, and also be useful as a long-juvenile allele, which causes late flowering under short-daylength conditions, in low-latitude regions.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1471-2229
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s12870-016-0704-9