Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase

Saburi, Wataru; Rachi-Otsuka, Hiroaki; Hondoh, Hironori; Okuyama, Masayuki; Mori, Haruhide; Kimura, Atsuo

Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase

Saburi, Wataru; Rachi-Otsuka, Hiroaki; Hondoh, Hironori et al.

Febs letters, 2015, 589(7), 865-869

Permalink : https://hdl.handle.net/2115/60921

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アクセス権	open access
URI	https://hdl.handle.net/2115/60921
タイトル	Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase
著者	Saburi, Wataru ; Rachi-Otsuka, Hiroaki ; Hondoh, Hironori ; Okuyama, Masayuki ; Mori, Haruhide ; Kimura, Atsuo
e-Rad_Researcher 00598089 著者名 Saburi, Wataru
著者名 Rachi-Otsuka, Hiroaki
著者名 Hondoh, Hironori
e-Rad_Researcher 00344490 著者名 Okuyama, Masayuki
e-Rad_Researcher 80241363 著者名 Mori, Haruhide
e-Rad_Researcher 90186312 著者名 Kimura, Atsuo
言語	英語
キーワード	Dextran glucosidase
	alpha-Glucosidase
	Oligo-1,6-glucosidase
	Substrate specificity
	Glycoside hydrolase family 13
発行日	2015-03-25
出版者	Elsevier
収録物名	Febs letters
巻(号)	589(7)
ページ	865-869
抄録	Glycoside hydrolase family 13 contains exo-glucosidases specific for alpha-(1 -> 4)- and alpha-(1 -> 6)-linkages including alpha-glucosidase, oligo-1,6-glucosidase, and dextran glucosidase. The alpha-(1 -> 6)-linkage selectivity of Streptococcus mutans dextran glucosidase was altered to alpha-(1 -> 4)-linkage selectivity through site-directed mutations at Val195, Lys275, and Glu371. V195A showed 1300-fold higher k(cat)/K-m for maltose than wild-type, but its k(cat)/K-m for isomaltose remained 2-fold higher than for maltose. K275A and E371A combined with V195A mutation only decreased isomaltase activity. V195A/K275A, V195A/E371A, and V195A/K275A/E371A showed 27-, 26-, and 73-fold higher k(cat)/K-m for maltose than for isomaltose, respectively. Consequently, the three residues are structural elements for recognition of the alpha-(1 -> 6)-glucosidic linkage. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 25728274
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2015.02.023