Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria

Fukuma, Naoki M.; Koike, Satoshi; Kobayashi, Yasuo

Archives of Microbiology, 2015, 197(2), 269-276

Permalink : https://hdl.handle.net/2115/61012

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アクセス権
open access
URI
https://hdl.handle.net/2115/61012
タイトル
Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria
著者
Fukuma, Naoki M. ; Koike, Satoshi ; Kobayashi, Yasuo
著者名
Fukuma, Naoki M.
e-Rad_Researcher
90431353
著者名
Koike, Satoshi
e-Rad_Researcher
50153648
著者名
Kobayashi, Yasuo
言語
英語
キーワード
Rumen bacteria
Fibrobacter succinogenes
Fiber digestion
Co-culture
mRNA expression
発行日
2015-03
出版者
Springer
収録物名
Archives of Microbiology
巻(号)
197(2)
ページ
269-276
抄録
Fibrobacter succinogenes is one of the most pivotal fibrolytic bacterial species in the rumen. In a previous study, we confirmed enhancement of fiber digestion in a co-culture of F. succinogenes S85 with non-fibrolytic ruminal strains R-25 and/or Selenomonas ruminantium S137. In the present study, mRNA expression level of selected functional genes in the genome of F. succinogenes S85 was monitored by real-time RT-PCR. Growth profile of F. succinogenes S85 was similar in both the monoculture and co-cultures with non-fibrolytics. However, expression of 16S rRNA gene of F. succinogenes S85 in the co-culture was higher (P < 0.01) than that of the monoculture. This finding suggests that metabolic activity of F. succinogenes S85 was enhanced by coexistence with strains R-25 and/or S. ruminantium S137. The mRNA expression of fumarate reductase and glycoside hydrolase genes was up-regulated (P < 0.01) when F. succinogenes S85 was co-cultured with non-fibrolytics. These results indicate the enhancement of succinate production and fiber hydrolysis by F. succinogenes S85 in co-cultures of S. ruminantium and R-25 strains.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The final publication is available at link.springer.com
PISSN
0302-8933
EISSN
1432-072X
NCID
AA00548209
関連情報
PMID
25354721
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s00203-014-1049-0