Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Nishida, Erika; Miyaji, Hirofumi; Kato, Akihito; Takita, Hiroko; Iwanaga, Toshihiko; Momose, Takehito; Ogawa, Kosuke; Murakami, Shusuke; Sugaya, Tsutomu; Kawanami, Masamitsu

Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Nishida, Erika; Miyaji, Hirofumi; Kato, Akihito et al.

International Journal of Nanomedicine, 2016, 11, 2265-2277

Permalink : https://hdl.handle.net/2115/61975

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アクセス権	open access
URI	https://hdl.handle.net/2115/61975
タイトル	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
著者	Nishida, Erika ; Miyaji, Hirofumi ; Kato, Akihito ; Takita, Hiroko ; Iwanaga, Toshihiko ; Momose, Takehito ; Ogawa, Kosuke ; Murakami, Shusuke ; Sugaya, Tsutomu ; Kawanami, Masamitsu
著者名 Nishida, Erika
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
e-Rad_Researcher 40507571 著者名 Kato, Akihito
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
著者名 Momose, Takehito
著者名 Ogawa, Kosuke
著者名 Murakami, Shusuke
e-Rad_Researcher 10211301 著者名 Sugaya, Tsutomu
e-Rad_Researcher 10133761 著者名 Kawanami, Masamitsu
言語	英語
キーワード	biocompatibility
	biomaterial
	bone tissue engineering
	cell ingrowth
	collagen sponge
	macrophage
	nanocarbon
発行日	2016-05-24
出版者	Dove Medical Press
収録物名	International Journal of Nanomedicine
巻(号)	11
ページ	2265-2277
抄録	Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 μg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 μg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 μg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 μg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 3.0 Unported
PISSN	1176-9114
EISSN	1178-2013
NCID	AA1215861X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2147/IJN.S104778