Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes

Kobayashi, Shintaro; Suzuki, Tadaki; Kawaguchi, Akira; Phongphaew, Wallaya; Yoshii, Kentaro; Iwano, Tomohiko; Harada, Akihiro; Kariwa, Hiroaki; Orba, Yasuko; Sawa, Hirofumi

Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes

Kobayashi, Shintaro; Suzuki, Tadaki; Kawaguchi, Akira et al.

Journal of Biological Chemistry (JBC), 2016, 291(12), 6559-6568

Permalink : https://hdl.handle.net/2115/62049

このアイテムのアクセス数:1,288件（2026-10-11 03:10 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
JBC 291 (12)p.6559-6568	pdf	1.05 MB	900

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/62049
タイトル	Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes
著者	Kobayashi, Shintaro ; Suzuki, Tadaki ; Kawaguchi, Akira ; Phongphaew, Wallaya ; Yoshii, Kentaro ; Iwano, Tomohiko ; Harada, Akihiro ; Kariwa, Hiroaki ; Orba, Yasuko ; Sawa, Hirofumi
e-Rad_Researcher 00634205 著者名 Kobayashi, Shintaro
e-Rad_Researcher 30527180 著者名 Suzuki, Tadaki
著者名 Kawaguchi, Akira
著者名 Phongphaew, Wallaya
e-Rad_Researcher 50421988 著者名 Yoshii, Kentaro
著者名 Iwano, Tomohiko
e-Rad_Researcher 40251441 著者名 Harada, Akihiro
e-Rad_Researcher 70224714 著者名 Kariwa, Hiroaki
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
言語	英語
キーワード	Rab
	vesicles
	transport
	Flavivirus
	infection
発行日	2016-03-18
出版者	American Society for Biochemistry and Molecular Biology (ASBMB)
収録物名	Journal of Biological Chemistry (JBC)
巻(号)	291(12)
ページ	6559-6568
抄録	West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This research was originally published in Journal of Biological Chemistry. Shintaro Kobayashi, Tadaki Suzuki, Akira Kawaguchi, Wallaya Phongphaew, Kentaro Yoshii, Tomohiko Iwano, Akihiro Harada, Hiroaki Kariwa, Yasuko Orba and Hirofumi Sawa. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes. The Journal of Biological Chemistry. 2016; 291(12): 6559-6568. © the American Society for Biochemistry and Molecular Biology.
PISSN	0021-9258
EISSN	1083-351X
NCID	AA00251083
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1074/jbc.M115.712760