Lactobacillus rhamnosus GG increases Toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo

Aoki-Yoshida, A.; Saito, S.; Fukiya, S.; Aoki, R.; Takayama, Y.; Suzuki, C.; Sonoyama, K.

Lactobacillus rhamnosus GG increases Toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo

Aoki-Yoshida, A.; Saito, S.; Fukiya, S. et al.

Beneficial microbes, 2016, 7(3), 421-429

Permalink : https://hdl.handle.net/2115/62352

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アクセス権	open access
URI	https://hdl.handle.net/2115/62352
タイトル	Lactobacillus rhamnosus GG increases Toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo
著者	Aoki-Yoshida, A. ; Saito, S. ; Fukiya, S. ; Aoki, R. ; Takayama, Y. ; Suzuki, C. ; Sonoyama, K.
著者名 Aoki-Yoshida, A.
著者名 Saito, S.
著者名 Fukiya, S.
著者名 Aoki, R.
著者名 Takayama, Y.
著者名 Suzuki, C.
著者名 Sonoyama, K.
言語	英語
キーワード	rotavirus
	intestinal organoids
	interferon-alpha
	CXCL1
	probiotics
発行日	2016-07-05
出版者	Wageningen Academic Publishers
収録物名	Beneficial microbes
巻(号)	7(3)
ページ	421-429
抄録	Administration of Lactobacillus rhamnosus GG (LGG) has been reported to be therapeutically effective against acute secretory diarrhoea resulting from the structural and functional intestinal mucosal lesions induced by rotavirus infection; however, the underlying mechanisms remain to be completely elucidated. Because Toll-like receptor 3 (TLR3) plays a key role in the innate immune responses following the recognition of rotavirus, the present study examined whether LGG influences TLR3 gene expression in murine small intestine ex vivo and in vivo. We employed cultured intestinal organoids derived from small intestinal crypts as an ex vivo tissue model. LGG supplementation increased TLR3 mRNA levels in the intestinal organoids, as estimated by quantitative real-time polymerase chain reaction. Likewise, single and 7-day consecutive daily administrations of LGG increased TLR3 mRNA levels in the small intestine of C57BL/6N mice. The mRNA levels of other TLRs were not substantially altered both ex vivo and in vivo. In addition, LGG supplementation increased the mRNA levels of an antiviral type 1 interferon, interferon-alpha (IFN-alpha), and a neutrophil chemokine, CXCL1, upon stimulation with a synthetic TLR3 ligand, poly(I:C) in the intestinal organoids. LGG administration did not alter IFN-alpha and CXCL1 mRNA levels in the small intestine in vivo. Supplementation of other bacterial strains, Bifidobacterium bifidum and Lactobacillus paracasei, failed to increase TLR3 and poly(I: C)-stimulated CXCL1 mRNA levels ex vivo. We propose that upregulation of TLR3 gene expression may play a pivotal role in the therapeutic efficacy of LGG against rotavirus-associated diarrhoea. In addition, we demonstrated that intestinal organoids may be a promising ex vivo tissue model for investigating host-pathogen interactions and the antiviral action of probiotics in the intestinal epithelium.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at http://www.wageningenacademic.com/doi/abs/10.3920/BM2015.0169.
PISSN	1876-2883
関連情報	PMID 27013459
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.3920/BM2015.0169