Strain differences in cytochrome P450 mRNA and protein expression, and enzymatic activity among Sprague Dawley, Wistar, Brown Norway and Dark Agouti rats

Nishiyama, Yoshihiro; Nakayama, Shouta M. M.; Watanabe, Kensuke P.; Kawai, Yusuke K.; Ohno, Marumi; Ikenaka, Yoshinori; Ishizuka, Mayumi

Strain differences in cytochrome P450 mRNA and protein expression, and enzymatic activity among Sprague Dawley, Wistar, Brown Norway and Dark Agouti rats

Nishiyama, Yoshihiro; Nakayama, Shouta M. M.; Watanabe, Kensuke P. et al.

Journal of Veterinary Medical Science, 2016, 78(4), 675-680

Permalink : https://hdl.handle.net/2115/62356

このアイテムのアクセス数:1,048件（2026-08-24 20:56 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
78_15-0299	pdf	653 KB	743

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/62356
タイトル	Strain differences in cytochrome P450 mRNA and protein expression, and enzymatic activity among Sprague Dawley, Wistar, Brown Norway and Dark Agouti rats
著者	Nishiyama, Yoshihiro ; Nakayama, Shouta M. M. ; Watanabe, Kensuke P. ; Kawai, Yusuke K. ; Ohno, Marumi ; Ikenaka, Yoshinori ; Ishizuka, Mayumi
著者名 Nishiyama, Yoshihiro
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
著者名 Watanabe, Kensuke P.
著者名 Kawai, Yusuke K.
著者名 Ohno, Marumi
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	cytochrome P450
	metabolism
	Rattus norvegicus
	regulation
	strain difference
発行日	2016-04
出版者	公益社団法人日本獣医学会
出版者	The Japanese Society of Veterinary Science
収録物名	Journal of Veterinary Medical Science
巻(号)	78(4)
ページ	675-680
抄録	Rat cytochrome P450 (CYP) exhibits inter-strain differences, but their analysis has been scattered across studies under different conditions. To identify these strain differences in CYP more comprehensively, mRNA expression, protein expression and metabolic activity among Wistar (WI), Sprague Dawley (SD), Dark Agouti (DA) and Brown Norway (BN) rats were compared. The mRNA level and enzymatic activity of CYP1A1 were highest in SD rats. The rank order of Cyp3a2 mRNA expression mirrored its protein expression, i.e., DA>BN>SD>WI, and was similar to the CYP3A2-dependent warfarin metabolic activity, i.e., DA>SD>BN>WI. These results suggest that the strain differences in CYP3A2 enzymatic activity are caused by differences in mRNA expression. Cyp2b1 mRNA levels, which were higher in DA rats, did not correlate with its protein expression or enzymatic activity. This suggests that the strain differences in enzymatic activity are not related to Cyp2b1 mRNA expression. In conclusion, WI rats tended to have the lowest CYP1A1, 2B1 and 3A2 mRNA expression, protein expression and enzymatic activity among the strains. In addition, SD rats had the highest CYP1A1 mRNA expression and activity, while DA rats had higher CYP2B1 and CYP3A2 mRNA and protein expression. These inter-strain differences in CYP could influence pharmacokinetic considerations in preclinical toxicological studies.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
EISSN	1347-7439
NCID	AA11510037
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.15-0299