Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent : a pilot study

Mizushima, Takeshi; Ohnishi, Shunsuke; Shimizu, Yuichi; Hatanaka, Yutaka; Hatanaka, Kanako C.; Hosono, Hidetaka; Kubota, Yoshimasa; Natsuizaka, Mitsuteru; Kamiya, Mako; Ono, Shouko; Homma, Akihiro; Kato, Mototsugu; Sakamoto, Naoya; Urano, Yasuteru

Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent : a pilot study

Mizushima, Takeshi; Ohnishi, Shunsuke; Shimizu, Yuichi et al.

BMC Cancer, 2016, 16, 411

Permalink : https://hdl.handle.net/2115/62385

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アクセス権	open access
URI	https://hdl.handle.net/2115/62385
タイトル	Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent : a pilot study
著者	Mizushima, Takeshi ; Ohnishi, Shunsuke ; Shimizu, Yuichi ; Hatanaka, Yutaka ; Hatanaka, Kanako C. ; Hosono, Hidetaka ; Kubota, Yoshimasa ; Natsuizaka, Mitsuteru ; Kamiya, Mako ; Ono, Shouko ; Homma, Akihiro ; Kato, Mototsugu ; Sakamoto, Naoya ; Urano, Yasuteru
著者名 Mizushima, Takeshi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
e-Rad_Researcher 90333608 著者名 Shimizu, Yuichi
e-Rad_Researcher 30589924 著者名 Hatanaka, Yutaka
著者名 Hatanaka, Kanako C.
著者名 Hosono, Hidetaka
著者名 Kubota, Yoshimasa
著者名 Natsuizaka, Mitsuteru
e-Rad_Researcher 90596462 著者名 Kamiya, Mako
著者名 Ono, Shouko
e-Rad_Researcher 30312359 著者名 Homma, Akihiro
e-Rad_Researcher 60271673 著者名 Kato, Mototsugu
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
著者名 Urano, Yasuteru
言語	英語
キーワード	Fluorescent imaging
	γ-Glutamyltranspeptidase
	Head and neck squamous cell carcinoma
発行日	2016-07-07
出版者	BioMed Central
収録物名	BMC Cancer
巻(号)	16
ページ	411
抄録	Background: Detecting superficial head and neck squamous cell carcinoma (HNSCC) by endoscopy is challenging because of limited morphological hallmarks, and iodine cannot be applied to head and neck lesions due to severe mucosal irritation. γ-glutamyltranspeptidase (GGT), a cell surface enzyme, is overexpressed in several cancers, and it has been reported that γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a fluorescent targeting agent which can be enzymatically activated and becomes fluorescent after cleavage of a GGT-specific sequence, can be activated within a few minutes after application to animal models. We investigated whether early HNSCC can be detected by applying gGlu-HMRG to clinical samples. Methods: gGlu-HMRG was applied to four HNSCC cell lines, and fluorescence was observed by fluorescence microscopy and flow cytometry. Immunohistological examination was performed in three recent cases of endoscopic submucosal dissection (ESD) to investigate GGT expression. Fluorescence imaging with gGlu-HMRG in eight clinical samples resected by ESD or surgery was performed, and fluorescence intensity of tumor and normal mucosa regions of interest (ROI) was prospectively measured. Results: All four gGlu-HMRG-applied cell lines emitted green fluorescence. Immunohistological examination demonstrated that GGT was highly expressed in HNSCC of the recent three ESD cases but barely in the normal mucosa. Fluorescence imaging showed that iodine-voiding lesions became fluorescent within a few minutes after application of gGlu-HMRG in all eight resected tumors. Tumor ROI fluorescence intensity was significantly higher than in the normal mucosa five minutes after gGlu-HMRG application. Conclusions: Fluorescence imaging with gGlu-HMRG would be useful for early detection of HNSCC.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
EISSN	1471-2407
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s12885-016-2421-z