Human Amnion-Derived Mesenchymal Stem Cell Transplantation Ameliorates Liver Fibrosis in Rats

Kubo, Kimitoshi; Ohnishi, Shunsuke; Hosono, Hidetaka; Fukai, Moto; Kameya, Ayano; Higashi, Ryosuke; Yamada, Takahiro; Onishi, Reizo; Yamahara, Kenichi; Takeda, Hiroshi; Sakamoto, Naoya

Human Amnion-Derived Mesenchymal Stem Cell Transplantation Ameliorates Liver Fibrosis in Rats

Kubo, Kimitoshi; Ohnishi, Shunsuke; Hosono, Hidetaka et al.

Transplantation Direct, 2015, 1(4), e16

Permalink : https://hdl.handle.net/2115/62386

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アクセス権	open access
URI	https://hdl.handle.net/2115/62386
タイトル	Human Amnion-Derived Mesenchymal Stem Cell Transplantation Ameliorates Liver Fibrosis in Rats
著者	Kubo, Kimitoshi ; Ohnishi, Shunsuke ; Hosono, Hidetaka ; Fukai, Moto ; Kameya, Ayano ; Higashi, Ryosuke ; Yamada, Takahiro ; Onishi, Reizo ; Yamahara, Kenichi ; Takeda, Hiroshi ; Sakamoto, Naoya
著者名 Kubo, Kimitoshi
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Hosono, Hidetaka
e-Rad_Researcher 60374344 著者名 Fukai, Moto
著者名 Kameya, Ayano
著者名 Higashi, Ryosuke
e-Rad_Researcher 20419948 著者名 Yamada, Takahiro
著者名 Onishi, Reizo
e-Rad_Researcher 50450888 著者名 Yamahara, Kenichi
e-Rad_Researcher 60261294 著者名 Takeda, Hiroshi
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
発行日	2015-05
出版者	Wolters Kluwer Health
収録物名	Transplantation Direct
巻(号)	1(4)
ページ	e16
抄録	Background: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine. Recently, several studies have shown that MSCs can be easily isolated from human amnion. In this study, we investigated the therapeutic effect of transplantation of human amnion-derived MSCs (hAMSCs) in rats with liver fibrosis. Methods: Liver fibrosis was induced by an intraperitoneal injection of 2 mL/kg of 50% carbon tetrachloride twice a week for 6 weeks. At 3 weeks, hAMSCs (1 × 10^6 cells) were transplanted intravenously. Rats were sacrificed at 7 weeks, and histological analyses and quantitative reverse-transcription polymerase chain reaction were performed. In vitro experiments were conducted to investigate the effect of hAMSCs on the activation of Kupffer cells. Results: Transplantation of hAMSCs significantly reduced the fibrotic area, deposition of type-I collagen, the number of α-smooth muscle actin-positive hepatic stellate cells, and CD68-positive Kupffer cells in the livers. messenger RNA expression of α-smooth muscle actin and tissue inhibitor of metalloproteinase-1 was significantly decreased and the expression of matrix metalloproteinase-9 and hepatocyte growth factor was significantly increased in the liver of hAMSC-treated rats. Transplantation of hAMSCs at 3 weeks plus 5 weeks did not have an additive effect. In vitro experiments demonstrated that Kupffer cell activation induced by lipopolysaccharide was significantly decreased by culturing with conditioned medium obtained from hAMSCs. Conclusions: Transplantation of hAMSCs provided significant improvement in a rat model of liver fibrosis, possibly through the inhibition of Kupffer cell and hepatic stellate cell activation. hAMSCs may be a potential new treatment for liver fibrosis.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	2373-8731
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1097/TXD.0000000000000525