In vivo imaging of clock gene expression in multiple tissues of freely moving mice

Hamada, Toshiyuki; Sutherland, Kenneth; Ishikawa, Masayori; Miyamoto, Naoki; Honma, Sato; Shirato, Hiroki; Honma, Ken-ichi

In vivo imaging of clock gene expression in multiple tissues of freely moving mice

Hamada, Toshiyuki; Sutherland, Kenneth; Ishikawa, Masayori et al.

Nature communications, 2016, 7, 11705

Permalink : https://hdl.handle.net/2115/62597

このアイテムのアクセス数:1,212件（2026-08-18 02:46 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Nat Commun_7_11705	pdf	2.62 MB	726

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/62597
タイトル	In vivo imaging of clock gene expression in multiple tissues of freely moving mice
著者	Hamada, Toshiyuki ; Sutherland, Kenneth ; Ishikawa, Masayori ; Miyamoto, Naoki ; Honma, Sato ; Shirato, Hiroki ; Honma, Ken-ichi
e-Rad_Researcher 20360208 著者名 Hamada, Toshiyuki
e-Rad_Researcher 30451446 著者名 Sutherland, Kenneth
e-Rad_Researcher 80314772 著者名 Ishikawa, Masayori
e-Rad_Researcher 00552879 著者名 Miyamoto, Naoki
e-Rad_Researcher 20142713 著者名 Honma, Sato
e-Rad_Researcher 20187537 著者名 Shirato, Hiroki
e-Rad_Researcher 40113625 著者名 Honma, Ken-ichi
言語	英語
発行日	2016-06-10
出版者	Nature
収録物名	Nature communications
巻(号)	7
ページ	11705
抄録	Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2041-1723
NCID	AA12645905
関連情報	PMID 27285820
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/ncomms11705