open access

https://hdl.handle.net/2115/62599

Post-translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet

英語

Blue Native polyacrylamide gel electrophoresis

2015-07-04

Wiley-blackwell

The Plant Journal

83(2)

290-299

Genetic conflict between cytoplasmically inherited elements and nuclear genes due to their different transmission patterns can be seen in cytoplasmic male sterility (CMS), the mitochondrion-encoded inability to shed functional pollen. CMS is associated with a mitochondrial ORF absent from non sterility-inducing mitochondria (S-orf). Nuclear genes that suppress CMS are called restorer-of-fertility (Rf) genes. Post-transcriptional and translational repression of S-orf mediates the molecular action of Rf that encodes a class of RNA-binding proteins having pentatricopeptide repeat (PPR) motifs. Besides the PPR-type of Rfs, there are also non-PPR Rfs, but the molecular interactions between non-PPR Rf and S-orf have not been described. In this study, we investigated the interaction of sugar beet bvORF20, a non-PPR Rf, with preSatp6, the sugar beet S-orf. Anthers expressing bvORF20 contained a protein that interacted with preSATP6 protein. Analysis of anthers and transgenic calli expressing a FLAG-tagged bvORF20 suggested binding of preSATP6 to bvORF20. To see the effect of bvORF20 on preSATP6, which exists as a 250-kDa protein-complex in CMS plants, signal bands of preSATP6 in bvORF20-expressing and non-expressing anthers were compared by immunoblotting combined with Blue Native polyacrylamide gel electrophoresis. The signal intensity of the 250-kDa band decreased significantly and 200- and 150-kDa bands appeared in bvORF20-expressing anthers. Transgenic callus expressing bvORF20 also generated the 200- and 150-kDa bands. The 200-kDa complex likely includes both preSATP6 and bvORF20. Post-translational interaction between preSATP6 and bvORF20 appears to alter the higher order structure of preSATP6 that may lead to fertility restoration in sugar beet.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: "Post-translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet", The Plant Journal, 83(2)pp290-299, which has been published in final form at 10.1111/tpj.12888. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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