Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells

Oasa, Sho; Sasaki, Akira; Yamamoto, Johtaro; Mikuni, Shintaro; Kinjo, Masataka

Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells

Oasa, Sho; Sasaki, Akira; Yamamoto, Johtaro et al.

FEBS Letters, 2015, 589(17), 2171-2178

Permalink : https://hdl.handle.net/2115/62623

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/62623
タイトル	Homodimerization of glucocorticoid receptor from single cells investigated using fluorescence correlation spectroscopy and microwells
著者	Oasa, Sho ; Sasaki, Akira ; Yamamoto, Johtaro ; Mikuni, Shintaro ; Kinjo, Masataka
著者名 Oasa, Sho
著者名 Sasaki, Akira
著者名 Yamamoto, Johtaro
著者名 Mikuni, Shintaro
e-Rad_Researcher 70177971 著者名 Kinjo, Masataka
言語	英語
キーワード	Dissociation constant
	Fluorescence correlation spectroscopy
	Glucocorticoid receptor
	Homodimerization
	Microwell
	Single cell
発行日	2015-08-04
出版者	WILEY
収録物名	FEBS Letters
巻(号)	589(17)
ページ	2171-2178
抄録	Glucocorticoid receptor alpha (GR) binds to the promoter regions of target genes as a homodimer and activates its transcriptional process. Though the homodimerization is thought to be the initial and essential process, the dissociation constant for homodimerization of GR remains controversial. To quantify homodimerization of (enhanced green fluorescence protein) EGFP-(glucocorticoid receptor) GR, the particle brightness in lysates from single cell was estimated for the fraction of homodimeric EGFP-GR using fluorescence correlation spectroscopy and microwells. Fitting the data with a bimolecular reaction model, the dissociation constant was determined. Moreover slow-diffusion complex was observed. These results suggest that EGFP-GR forms not only a monomer-dimer equivalent state but also a large-molecular-weight complex.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: FEBS Letters Vol. 589 (17) p.2171-2178 2015, which has been published in final form at 10.1016/j.febslet.2015.07.003. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN	0014-5793
関連情報	PMID 26183204
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2015.07.003