open access

https://hdl.handle.net/2115/62683

Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs

英語

dogs

2010-10

Wiley-Blackwell

Journal of Periodontal Research

45(5)

658-663

Background and objective: Modification of the root surface may play an important role in regenerating the periodontal attachment between the root and periodontal connective tissue. We speculated that bone morphogenetic protein (BMP) application to root surface resulted in a novel attachment by cementum-like hard tissue, although non-osteogenic tissue proliferated to the root surface. The aim of this study was to examine whether BMP-2 guided cementum-like tissue deposition on a BMP-conditioned root surface. Material and Methods: Root dentin on the buccal side of 24 teeth in four beagle dogs was surgically exposed. The denuded root dentin surfaces were demineralized with EDTA and washed with saline. Subsequently, 15 μl of BMP-2 solution (loading dose; 0.4 and 1.0 μg/μl) was applied to the root dentin surface. In the control, phosphate-buffered saline was applied to the root surface. Specimens were histologically analyzed 16 weeks after surgery. Results: Formation of cementum-like tissue was frequently observed on the BMP-2-conditioned root at the coronal portion. Cellular cementum-like tissue was separated from the original cementum and encapsulated with gingival connective tissue. Cementum-like tissue formation with BMP at 1.0 μg/μl was significantly greater than those in the control and BMP at 0.4 μg/μl. Downgrowth of the junctional epithelium in the 1.0 μg/μl BMP group was significantly less than that in the control. Conclusions: Root dentin surface conditioning with BMP-2 stimulated cementum-like tissue formation and inhibited epithelial downgrowth.

学術雑誌論文

Accepted Manuscript

"This is the peer reviewed version of the following article: [Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs], which has been published in final form at [http://dx.doi.org/10.1111/j.1600-0765.2010.01281.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."

0022-3484

1600-0765

AA00704381