open access

https://hdl.handle.net/2115/62689

The Sam68 nuclear body is composed of two RNase-sensitive substructures joined by the adaptor HNRNPL

英語

2016-07-04

Rockefeller University Press

Journal of cell biology

214(1)

45-59

The mammalian cell nucleus contains membraneless suborganelles referred to as nuclear bodies (NBs). Some NBs are formed with an architectural RNA (arcRNA) as the structural core. Here, we searched for new NBs that are built on unidentified arcRNAs by screening for ribonuclease (RNase)-sensitive NBs using 32,651 fluorescently tagged human cDNA clones. We identified 32 tagged proteins that required RNA for their localization in distinct nuclear foci. Among them, seven RNA-binding proteins commonly localized in the Sam68 nuclear body (SNB), which was disrupted by RNase treatment. Knockdown of each SNB protein revealed that SNBs are composed of two distinct RNase-sensitive substructures. One substructure is present as a distinct NB, termed the DBC1 body, in certain conditions, and the more dynamic substructure including Sam68 joins to form the intact SNB. HNRNPL acts as the adaptor to combine the two substructures and form the intact SNB through the interaction of two sets of RNA recognition motifs with the putative arcRNAs in the respective substructures.

学術雑誌論文

Version of Record

Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported

©Mannen et al., 2016. Originally published in the journal of cell biology. doi:10.1083/jcb.201601024

0021-9525

AA00694812