Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b

Fukuda, Miki; Kaneko, Nobuto; Kawaguchi, Kohei; Hevroy, Ernst M.; Hara, Akihiko; Shimizu, Munetaka

Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b

Fukuda, Miki; Kaneko, Nobuto; Kawaguchi, Kohei et al.

Comparative biochemistry and physiology A molecular & integrative physiology, 2015, 187, 66-73

Permalink : https://hdl.handle.net/2115/62749

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アクセス権	open access
URI	https://hdl.handle.net/2115/62749
タイトル	Development of a time-resolved fluoroimmunoassay for salmon insulin-like growth factor binding protein-1b
著者	Fukuda, Miki ; Kaneko, Nobuto ; Kawaguchi, Kohei ; Hevroy, Ernst M. ; Hara, Akihiko ; Shimizu, Munetaka
著者名 Fukuda, Miki
著者名 Kaneko, Nobuto
著者名 Kawaguchi, Kohei
著者名 Hevroy, Ernst M.
e-Rad_Researcher 40091483 著者名 Hara, Akihiko
e-Rad_Researcher 90431337 著者名 Shimizu, Munetaka
言語	英語
キーワード	Fasting
	Growth
	Immunoassay
	Insulin-like growth factor binding protein
	Salmon
	Smoltification
発行日	2015-09
出版者	Elsevier
収録物名	Comparative biochemistry and physiology A molecular & integrative physiology
巻(号)	187
ページ	66-73
抄録	In salmon plasma/serum, three major insulin-like growth factor binding proteins (IGFBPs) are consistently detected at 22-, 28- and 41-kDa. The 22-kDa form has been identified as IGFBP-1b and shown to increase under catabolic conditions. We developed a competitive time-resolved fluoroimmunoassay (TR-FIA) for salmon IGFBP-1b. Purified salmon IGFBP-1b was used for biotin-labeling, assay standard and antiserum production. The TR-FIA did not cross-react with the 41-kDa form (IGFBP-2b) but showed 3% cross-reactivity with the 28-kDa form (IGFBP-1a). It measured IGFBP-1b levels as low as 0.4 ng/ml, and ED80 and ED20 were 0.9 and 24.6 ng/ml, respectively. There appears to be little interference by IGF-I. Using the TR-FIA, serum IGFBP-1b levels were measured in individually-tagged underyearling masu salmon fed or fasted for 5 weeks, or fasted for 3 weeks followed by refeeding for 2 weeks. Fasting for 3 weeks significantly increased circulating IGFBP-1b levels, while it returned to the basal levels after prolonged fasting for additional 2 weeks. Serum IGFBP-1b level negatively correlated with body weight, condition factor, specific growth rate and serum IGF-I level. During parr-smolt transformation of masu salmon, average circulating IGFBP-1b levels were the highest in May. There was a positive correlation between serum IGFBP-1b and IGF-I, which is in contrast to that in the fasting/feeding experiment. IGFBP-1b also showed a positive relationship with gill Na+, K+-ATPase activity. These results suggest that the relationship between circulating IGFBP-1b and IGF-I during smoltification differs from that during fasting and IGFBP-1b may play a role in the development of hypoosmoregulatory ability. (C) 2015 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1095-6433
NCID	AA11183804
関連情報	PMID 25960447
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.cbpa.2015.04.016