Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium

Wang, Wenshuang; Han, Wenjun; Cai, Xingya; Zheng, Xiaoyu; Sugahara, Kazuyuki; Li, Fuchuan

Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium

Wang, Wenshuang; Han, Wenjun; Cai, Xingya et al.

Journal of Biological Chemistry, 2015, 290(12), 7823-7832

Permalink : https://hdl.handle.net/2115/62913

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アクセス権	open access
URI	https://hdl.handle.net/2115/62913
タイトル	Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium
著者	Wang, Wenshuang ; Han, Wenjun ; Cai, Xingya ; Zheng, Xiaoyu ; Sugahara, Kazuyuki ; Li, Fuchuan
著者名 Wang, Wenshuang
著者名 Han, Wenjun
著者名 Cai, Xingya
著者名 Zheng, Xiaoyu
e-Rad_Researcher 60154449 著者名 Sugahara, Kazuyuki
著者名 Li, Fuchuan
言語	英語
キーワード	Chondroitin Sulfate
	Dermatan Sulfate
	Glycosaminoglycan
	Oligosaccharide
	Polysaccharide
	Endosulfatase
	Marine Bacterium
	Sulfated Polysaccharide
発行日	2015-03-20
出版者	American Society for Biochemistry and Molecular Biology (ASBMB)
収録物名	Journal of Biological Chemistry
巻(号)	290(12)
ページ	7823-7832
抄録	Sulfatases are potentially useful tools for structure-function studies of glycosaminoglycans (GAGs). To date, various GAG exosulfatases have been identified in eukaryotes and prokaryotes. However, endosulfatases that act on GAGs have rarely been reported. Recently, a novel HA and CS lyase (HCLase) was identified for the first time from a marine bacterium (Han, W., Wang, W., Zhao, M., Sugahara, K., and Li, F. (2014) J. Biol. Chem. 289, 27886–27898). In this study, a putative sulfatase gene, closely linked to the hclase gene in the genome, was recombinantly expressed and characterized in detail. The recombinant protein showed a specific N-acetylgalactosamine-4-O-sulfatase activity that removes 4-O-sulfate from both disaccharides and polysaccharides of chondroitin sulfate (CS)/dermatan sulfate (DS), suggesting that this sulfatase represents a novel endosulfatase. The novel endosulfatase exhibited maximal reaction rate in a phosphate buffer (pH 8.0) at 30 °C and effectively removed 17–65% of 4-O-sulfates from various CS and DS and thus significantly inhibited the interactions of CS and DS with a positively supercharged fluorescent protein. Moreover, this endosulfatase significantly promoted the digestion of CS by HCLase, suggesting that it enhances the digestion of CS/DS by the bacterium. Therefore, this endosulfatase is a potential tool for use in CS/DS-related studies and applications.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	This research was originally published in Journal of Biological Chemistry. Wenshuang Wang, Wenjun Han, Xingya Cai, Xiaoyu Zheng, Kazuyuki Sugahara and Fuchuan Li. Cloning and Characterization of a Novel Chondroitin Sulfate/Dermatan Sulfate 4-O-Endosulfatase from a Marine Bacterium. Journal of Biological Chemistry. 2015; Vol:290(12) p.7823-7832 © the American Society for Biochemistry and Molecular Biology
PISSN	0021-9258
EISSN	1083-351X
関連情報	PMID 25648894
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1074/jbc.M114.629154