Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

OKABE, Hiroaki; HUI, Shu-Ping; FUDA, Hirotoshi; FURUKAWA, Takayuki; TAKEDA, Seiji; SHRESTHA, Rojeet; MIURA, Yusuke; WATANABE, Mitsugu; CHIBA, Hitoshi

Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)

OKABE, Hiroaki; HUI, Shu-Ping; FUDA, Hirotoshi et al.

Analytical Sciences, 2015, 31(12), 1341-1344

Permalink : https://hdl.handle.net/2115/62970

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アクセス権	open access
URI	https://hdl.handle.net/2115/62970
タイトル	Mass Spectrometric Quantification of Amphipathic, Polyphenolic Antioxidant of the Pacific Oyster (Crassostrea Gigas)
著者	OKABE, Hiroaki ; HUI, Shu-Ping ; FUDA, Hirotoshi ; FURUKAWA, Takayuki ; TAKEDA, Seiji ; SHRESTHA, Rojeet ; MIURA, Yusuke ; WATANABE, Mitsugu ; CHIBA, Hitoshi
著者名 OKABE, Hiroaki
著者名 HUI, Shu-Ping
著者名 FUDA, Hirotoshi
著者名 FURUKAWA, Takayuki
著者名 TAKEDA, Seiji
著者名 SHRESTHA, Rojeet
著者名 MIURA, Yusuke
著者名 WATANABE, Mitsugu
e-Rad_Researcher 70197622 著者名 CHIBA, Hitoshi
言語	英語
キーワード	Amphipathicity
	polyphenol
	LC-MS/MS
	antioxidant
	Pacific oyster
	polar end-capped ODS
発行日	2015-12
出版者	日本分析化学会
出版者	Japan Society for Analytical Chemistry
収録物名	Analytical Sciences
巻(号)	31(12)
ページ	1341-1344
抄録	A novel amphipathic phenolic compound, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), that can be isolated from the Pacific oyster (Crassostrea gigas) has been found to protect human hepatocytes against oxidative stress. This study aims to establish a method for the measurement of DHMBA for industrial application. Liquid chromatography-tandem mass spectrometry using deuterated DHMBA as an internal standard and a polar end-capped ODS (Hypersil GOLD aQ) as the solid phase was validated. The limit of detection was 0.04 pmol (S/N = 5), and the limit of quantitation was 0.1 pmol (S/N = 10). The calibration curve was linear throughout the range of 0.1 - 16 pmol (r(2) = 0.9995). This method successfully quantified DHMBA in oysters from 11 sea areas in Japan. The results showed that the yield of DHMBA was variable from 9.8 to 58.8 μg g(-1) whole oyster meat wet weight but not affected by the seawater temperature. The proposed LC-MS/MS method is useful in quantitative studies for DHMBA and potentially for other amphipathic substances.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0910-6340
EISSN	1348-2246
NCID	AA10500785
関連情報	PMID 26656828
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2116/analsci.31.1341