Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes

Ma, Yi-Shing; Yoshida, Shigeru; Kobayashi, Yu; Kawanishi, Noriaki; Furukawa, Takayuki; Fuda, Hirotoshi; Hui, Shu-Ping; Chiba, Hitoshi

Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes

Ma, Yi-Shing; Yoshida, Shigeru; Kobayashi, Yu et al.

Journal of Food and Nutrition Research, 2016, 4(8), 498-507

Permalink : https://hdl.handle.net/2115/62976

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アクセス権	open access
URI	https://hdl.handle.net/2115/62976
タイトル	Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes
著者	Ma, Yi-Shing ; Yoshida, Shigeru ; Kobayashi, Yu ; Kawanishi, Noriaki ; Furukawa, Takayuki ; Fuda, Hirotoshi ; Hui, Shu-Ping ; Chiba, Hitoshi
著者名 Ma, Yi-Shing
e-Rad_Researcher 20431314 著者名 Yoshida, Shigeru
著者名 Kobayashi, Yu
著者名 Kawanishi, Noriaki
e-Rad_Researcher 30754642 著者名 Furukawa, Takayuki
著者名 Fuda, Hirotoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
e-Rad_Researcher 70197622 著者名 Chiba, Hitoshi
言語	英語
キーワード	fatty acid uptake
	polyphenol
	mitochondrial respiration
	electrical stimulation
	C2C12 myotubes
発行日	2016
出版者	Science & Education Publishing
収録物名	Journal of Food and Nutrition Research
巻(号)	4(8)
ページ	498-507
抄録	Anti-oxidative effects of the Pacific oyster-derived phenolic antioxidant, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), has been documented in hepatocytes. Additionally, DHMBA-rich oyster extracts significantly attenuated obesity in a non-alcoholic steatohepatitis mouse model. Whether the administration of DHMBA might improve muscular mitochondrial function was investigated. The mouse C2C12-derived myotubes were loaded with oleic acid (400μM) and cultured for 24 hours in the presence of DHMBA (500μM) with or without electrical stimulation (ES), where ES was given as exercise mimic. The fatty acid uptake, lipid accumulation, and mitochondrial function were subsequently accessed. DHMBA and ES increased fatty acid uptake, TG contents, mitochondrial membrane potential, intracellular level of H2O2, and mitochondrial O2 consumption rate. Intracellular ATP content was significantly increased when both DHMBA and ES were loaded at the same time, suggesting their synergic action. Phosphorylated AMPKα, AMPKβ1, and acetyl-CoA carboxylase were increased by DHMBA, indicating a possible role for DHMBA for activation of metabolic adaptation system and consequent increase of fatty acid oxidation. In conclusion, DHMBA solely or in collaboration with exercise might possibly serve as a fitness food for obese persons by stimulating muscular fatty acid utilization and mitochondrial energy production. This assumption must be verified by animal experiment.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2333-1119
EISSN	2333-1240
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.12691/jfnr-4-8-3