An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas

Yamamichi, Akane; Kasama, Toshihiro; Ohka, Fumiharu; Suzuki, Hiromichi; Kato, Akira; Motomura, Kazuya; Hirano, Masaki; Ranjit, Melissa; Chalise, Lushun; Kurimoto, Michihiro; Kondo, Goro; Aoki, Kosuke; Kaji, Noritada; Tokeshi, Manabu; Matsubara, Toshio; Senga, Takeshi; Kaneko, Mika K.; Suzuki, Hidenori; Hara, Masahito; Wakabayashi, Toshihiko; Baba, Yoshinobu; Kato, Yukinari; Natsume, Atsushi

An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas

Yamamichi, Akane; Kasama, Toshihiro; Ohka, Fumiharu et al.

Science and Technology of Advanced Materials, 2016, 17(1), 618-625

Permalink : https://hdl.handle.net/2115/63209

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アクセス権	open access
URI	https://hdl.handle.net/2115/63209
タイトル	An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
著者	Yamamichi, Akane ; Kasama, Toshihiro ; Ohka, Fumiharu ; Suzuki, Hiromichi ; Kato, Akira ; Motomura, Kazuya ; Hirano, Masaki ; Ranjit, Melissa ; Chalise, Lushun ; Kurimoto, Michihiro ; Kondo, Goro ; Aoki, Kosuke ; Kaji, Noritada ; Tokeshi, Manabu ; Matsubara, Toshio ; Senga, Takeshi ; Kaneko, Mika K. ; Suzuki, Hidenori ; Hara, Masahito ; Wakabayashi, Toshihiko ; Baba, Yoshinobu ; Kato, Yukinari ; Natsume, Atsushi
著者名 Yamamichi, Akane
著者名 Kasama, Toshihiro
著者名 Ohka, Fumiharu
著者名 Suzuki, Hiromichi
著者名 Kato, Akira
著者名 Motomura, Kazuya
著者名 Hirano, Masaki
著者名 Ranjit, Melissa
著者名 Chalise, Lushun
著者名 Kurimoto, Michihiro
著者名 Kondo, Goro
著者名 Aoki, Kosuke
e-Rad_Researcher 90402479 著者名 Kaji, Noritada
e-Rad_Researcher 60311437 著者名 Tokeshi, Manabu
著者名 Matsubara, Toshio
著者名 Senga, Takeshi
著者名 Kaneko, Mika K.
著者名 Suzuki, Hidenori
著者名 Hara, Masahito
著者名 Wakabayashi, Toshihiko
e-Rad_Researcher 30183916 著者名 Baba, Yoshinobu
著者名 Kato, Yukinari
著者名 Natsume, Atsushi
言語	英語
キーワード	Glioma
	isocitrate dehydrogenase 1 mutation
	immuno-wall microdevice
	rapid diagnosis
	precision medicine
発行日	2016-10
出版者	Taylor & Francis
収録物名	Science and Technology of Advanced Materials
巻(号)	17(1)
ページ	618-625
抄録	World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1468-6996
EISSN	1878-5514
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1080/14686996.2016.1227222